Thermo Fisher, L3000150, Lipofectamine™ 3000 Transfection Reagent, 15 mL, 15 mL

Name: Lipofectamine™ 3000 Transfection Reagent, 15 mL, 15 mL
Brand: Thermo Fisher
SKU: L3000150

Quantity: 15 mL, Unit Size: Each, Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Catalog Number: L3000150

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Category

Lipid-Based Transfection Reagents

Pack Size15 mL

TagsTransfection Reagents Biochemical Reagents Nucleic Acid Introduction Reagents

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 15 mL
Unit Size: Each
This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfectionEfficiently Transfect Difficult-to-Transfect CellsLipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:Fibroblast (3T3, COS-7)Myoblast (C2C12, L6 CRL-1458)Hepatocyte (HepG2, HuH7)Erythroleukemic cell (K562)Breast carcinoma (MCF7, Hs578T)Prostate carcinoma (LNCap)Lung carcinoma (A549, NCI-H460)Osteosarcoma (U-2 OS, Saos-2)Colon carcinoma (Caco2, SW480)Pancreatic carcinoma (PANC-1)Skin melanoma (SK-MEL-28)Transfect Common Cells Efficiently and Cost-EffectivelyFor common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates).Achieve Superior Transfection Efficiency While Maintaining Low ToxicityLipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.Enable High Transfection Efficiency in Novel Genome Editing ApplicationsLipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Specifications

General

Classification	Animal Origin-Free
For Use With (Application)	Transfection
Product Line	Lipofectamine
Product Type	Transfection Reagent
Quantity	15 mL
Serum Compatible	Yes
Cell Type	Hard-to-Transfect Cells
Format	6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type	RNAi Plasmids (shRNA, miR)
Transfection Technique	Lipid-based Transfection
Unit Size	Each

Ordering

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