Thermo Fisher, R37601, LIVE/DEAD™ Cell Imaging Kit (488/570)

Name: LIVE/DEAD™ Cell Imaging Kit (488/570)
Brand: Thermo Fisher
SKU: R37601

Quantity: 1 mL kit, Unit Size: Each

Catalog Number: R37601

Pricing

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Category

Kidney Function Reagents

Pack Size1 mL

TagsCell Analysis Products Cell Based Assays Cell Function Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 mL kit
Unit Size: Each
• Allows discrimination between live and dead cells with two probes that measure recognized parameters of cytotoxicity and cell viability: intracellular esterase activity and plasma membrane integrity
• Based on cell-permeable dye for staining of live cells and cell-impermeable dye for staining of dead and dying cells
• Adapted for imaging platforms by optimizing for common green and red imaging filters used with FITC and Texas Red
• Live cell component produces intense, uniform green fluorescence in live cells (ex/em 488 nm/515nm
• Dead cell component produces predominantly nuclear red fluorescence (ex/em 570nm/602nm) in cells with compromised cell membranes, strong indicator of cell death and cytotoxicity
• Sensitive detection of key measures of cell health: viability, live/dead ratio, and cytotoxicity
• Ease of use with minimal dilutions
• Live cells are distinguished by presence of ubiquitous intracellular esterase activity as determined by the enzymatic conversion of virtually nonfluorescent cell-permeant calcein AM to intensely fluorescent calcein, which is well-retained within live cells
• Red component is cell-impermeant and therefore only enters cells with damaged membranes
• In dying and dead cells bright red fluorescence is generated upon binding to DNA
• Background fluorescence levels are inherently low with this assay technique because dyes are virtually nonfluorescent before interacting with cellsCell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Specifications

General

Cell Type	Mammalian Cells
Description	LIVE/DEAD™ Cell Imaging Kit (488/570)
Detection Method	Fluorescence
Dye Type	Other Label(s) or Dye(s)
Form	Frozen
Format	Tube(s)
Incubation Time	15 min
Quantity	1 mL kit
Shipping Condition	Dry Ice
Color	Red, Green
Emission	515/602
Excitation Wavelength Range	488, 570 nm
For Use With (Application)	Viability Assay
For Use With (Equipment)	Floid Cell Imaging System
Product Line	LIVE/DEAD, Molecular Probes
Product Type	Cell Imaging Kit
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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