Description
Wells: 20-well
Unit Size: 1 pack
The NuPAGE Tris-Acetate Midi Welcome Pack contains:• SureLock Tandem Midi Gel Tank (STM1001)• NuPAGE Tris-Acetate Midi Gels (2 boxes, 20 gels)• NuPAGE Tris-Acetate SDS Running Buffer, 20X (LA0041)• NuPAGE LDS Sample Buffer, 4X (NP0007)• NuPAGE Sample Reducing Agent, 10X (NP0009)• HiMark Pre-stained Protein Standard (LC5699)About the SureLock Tandem Midi Gel TankThe SureLock Tandem Midi Gel Tank provides for rapid electrophoresis of midi gels using minimal buffer (∼520 mL/gel) in a leak-free system. With a setup time of ∼30 seconds, the tank efficiently runs midi gels while also providing consistent performance. The tank features two independent chambers, allowing electrophoresis of one or two gels at a time, which saves on buffer and limits waste. The tank is compatible with all Invitrogen pre-cast midi gels and midi gel cassettes.About NuPAGE Tris-Acetate Midi GelsNuPAGE Tris-Acetate Midi protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate Midi gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional Laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine midi SDS-PAGE gels.Transfer and detectionFor transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP0006) for traditional wet transfer using the SureLock Tandem Midi Blot Module (STM2001). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).
