Wako, 010-21866, Anti 6×Histidine, Monoclonal Antibody(9F2)

This product is used for detection of C terminal 6His fusion recombinant protein by using immunoprecipitation and western blot., This product is for research use only. Do not administer it to human., The 6xhistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein., Monoclonal Anti 6xhistidine (6xHis) antibody was produced by hybridoma clone 9F2. This clone was established by fusing mouse myeloma cells and lymphocytes of mice immunized with synthetic 6xHis peptide that was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites., [Concentration], Indicated on the label., [Formulation], 1xPBS aqueous solution with 50% glycerol., [Clone No.], 9F2, [Subclass], IgG, 1, [Specificity], Specific for C-terminal of 6xHis tagged recombinant protein., [Working Concentration], Western blot 0.2 - 2 µg/mL, Immunoprecipitation 2 - 3 µg/20 µL Protein G beads slurry