This product is for research use only. Do not administer it to human., Argonaute2 (AGO2) was isolated as one of the main components of RISC (RNA-induced silencing complex). AGO2 captures siRNA and microRNA which are working as a guide molecule for interaction with target mRNAs in RNAi pathway. In this pathway, AGO2 catalyzes the nicking of target mRNAs and binding between RISC and target mRNAs., This monoclonal antibody is not only used for western blot and immunohistochemistry, but also immunoprecipitation of hAGO2., Monoclonal Anti human AGO2 antibody is produced by hybridoma clone 4G8, established by fusing mouse myeloma cells P3U1 and lymphocytes of BALB/c mice immunized with recombinant human AGO2 produced in, E. coli, This antibody was purified by an affinity column from cell culture supernatant., [Concentration], Indicated on each label., [Formulation], 1 x TBS (pH 7.4) aqueous solution with 10% glycerol containing 0.09% sodium azide, [Clone No.], 4G8, [Specificity], Specific to human AGO2, [Subclass], IgG, 1, [Working Dilution], Western blot 1:100 ~ 1:200, Immunoprecipitation 10 μg/20 μL 10% Protein G beads slurry, Immunocytochemistry 1:20 ~ 1:50, [Note], Avoid repeated freezing and thawing., [Product List for microRNA Research], Description, Wako Cat. # (Pkg. Size), microRNA Purification kits based on Immunoprecipitation method using antibody against Argonaute protein, microRNA Isolation Kit,, Human/Mouse, Ago1, 291-70201 (10 reactions), microRNA Isolation Kit,, Human, Ago2, 292-66701 (10 reactions), microRNA Isolation Kit,, Mouse, Ago2, 292-67301 (10 reactions), microRNA Isolation Kit,, Human, Ago3, 297-70301 (10 reactions), Preparation kits of, cDNA, encoding microRNA, microRNA Cloning, Kit Wako, 290-66501 (8 reactions), Single Strand DNA Ligase, thermostable, recombinant, Solution, 298-65103 (200 units); 292-65101 (500 units), Target, mRNA Cloning, Kit Wako, 298-67901 (30 reactions), PCR Purification Kit Wako, 298-68001 (10 reactions), THUNDER Taq Gold DNA Polymerase (containing Mg, 2+, free buffer), 317-07081 (250 U), Antibodies, Anti Ago1, Monoclonal Antibody (1F2), 018-22401 (50 μL), Anti Ago1, Monoclonal Antibody (2A7), 015-22411 (50 μL), Anti, Human, Ago2, Monoclonal Antibody (4G8), 011-22033 (50 μL); 015-22031 (100 μL), Anti Mouse Ago2, Monoclonal Antibody (2D4), 014-22023 (50 μL); 018-22021 (100 μL), Anti, Human, Ago3, Monoclonal Antibody, available soon!, Wako Cat. # (Pkg. Size), 1) For IP, Western Blot, 2) Cross reactivity with AGO2 protein of rat and hamster, 3) For purification of RNA captured by RISC
