Wako, 011-23096, Anti 6×Histidine, Monoclonal Antibody(9C11)

The 6xHistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein., Monoclonal anti 6xHistidine (6xHis) antibody was produced by hybridoma clone 9C11. This clone was established by fusing mouse myeloma cells and spleen cells of mice immunized with synthetic 6xHis peptide that was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites., This product is for laboratory use only ; use in any such application is the responsibility of the user., This product is for research use only. Do not administer it to human., [Concentration], Indicated on the label., [Formulation], 1 x PBS aqueous solution with 50% glycerol., [Clone no.], 9C11, [Subclass], IgG, 1, [Specificity], Specific for N and C terminal of 6 x His tagged recombinant protein., [Working Dilution], Western blot 1:1,000 - 1:10,000, Immunoprecipitation 2 - 10 μg/20 μL Protein G beads slurry