Wako, 012-22541, Anti Green Fluorescent Protein, Monoclonal Antibody(mFX75)

This product is for research use only. Do not administer it to human., Green fluorescent protein (GFP) from the jellyfish, Aequorea victoria, has been used with increasing success as a marker protein for gene expression, intracellular processes and localization ranging from bacteria to transgenic mice. Wako offers a mouse monoclonal antibody for Western blotting of GFP., [Concentration], Displayed on label., [Storage Buffer], D-PBS(-), 10 mmol/L Hepes (pH 7.4) and 10 mmol/L Sodium Azide., [Preparation], This monoclonal antibody is produced by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the GFP's partial peptide coupled with BSA after being immunized with GFP. The hybridoma supernatant cultured by Hollow fiber instrument was purified by Protein A affinity chromatography and prepared in D-PBS(-) solution containing 10 mmol Hepes (pH 7.4) and 10 mmol/L NaN, 3, ., [Clone No.], mFX75, [Subclass], IgG, 2a·κ, [Working Dilution], Western blotting 1:500 - 1:1,000, [Reference], 1. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G. and Cormier, M. J .:, Gene, ,, 111, , 229 (1992).