Wako, 018-20463, Anti Green Fluorescent Protein, Monoclonal Antibody

The green fluorescent protein (GFP) from the jellyfish Aequorea victria has been used with increasing success as a marker protein for gene expression, intracellular processes and localization ranging from bacteria to transgenic mice.Wako offers a mouse monoclonal antibody for immunoprecipitation or immunohistochemistry of native GFP and GFP variants., Concentration:, Shown on label, phosphate-buffered saline (PBS), pH 7.4 containing 10 mmol/L HEPES and 10 mmol/L sodium azide., Preparation:, This monoclonal antibody is produced by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the GFP'spartial peptide coupled with BSA after being immunized with GFP. The hybridoma supernatant cultured by protein free culture was subjected to ultrafiltration with an ultra-filter 30,000-molecular-weight cutoff and prepared in PBS solution containing 10 mmol/L HEPES and 10 mmol/L sodiumazide., Clone No.:, mFX73, Subclass:, IgG2a.k, Specificity:, Specific for Native GFP and its variant from jellyfish Aequorea victria. Denatured protein lacks antigen determinants., Working Concentration:, Immunoprecipitation, 1-5 micrograms / 5 microliter Sepharose, Immunohistochemistry, 0.5-1 micrograms / mL, References:, Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G. and Cormier, M. J.:, Gene, ,, 111, , 229 (1992)., Zhen, M. and Jin, Y. :, Nature, ,, 401, , 371 (1999)., Nakagawa, M., Fukata, M., Yamaga, M., Itoh, N. and Kaibuchi, K. :, J. Cell Sci., ,, 114, , 1829 (2001)., Fukata, M., Nakagawa, M., Itoh, N., Kawajiri, A., Yamaga, M., Kuroda, S. and Kaibuchi, K. :, Mol. Cell Biol., ,, 21, , 2165 (2001)., This product is for research use only. Do not administer it to human.