Wako, 205-13001, 0.2mol/L Tris-HCl Buffer Solution (pH 8.0)

, Endotoxin is a component of the outer membrane of Gram-negative bacteria and is a lipopolysaccharide (LPS). Since endotoxin causes serious symptoms such as fever and shock in the human body, the bacterial endotoxin test for pharmaceuticals, especially parenteral drugs, is important. Since J. Levin and F. B. Bang discovered that endotoxin gels an extract from horseshoe crab amebocytes (Limulus amebocyte lysate, or LAL), bacterial endotoxin detection by the Limulus test has been widely used., This method, known as the limulus test, was adopted in the US Pharmacopoeia in 1980, and later in 1988 in the Japanese Pharmacopoeia as well., , A buffer used for dissolving LAL and synthetic chromogenic substrates as described in JIS K8008 (General Rules for Biochemical Reagents)., pH 8.0