Wako, 295-50201, DNA ExtractorR Kit

This is for research use only. Do not administer it to human. Molecular Biology-DNA Extraction Kits. Detects and quantitates contaminant DNA in serum and residual DNA in biopharmaceuticals. Employs a new extraction procedure for DNA purification from a single tube. This procedure, using Sodium Iodide (NaI) as a chaotropic agent, realizes DNA isolation of both high quality and high recovery from biological fluids without the use of phenol or chloroform. Complex and laborious manipulations are avoided when using this kit. In addition, this kit has a modified application for use with Molecular Devices' Threshold(TM) System., PRINCIPLE of DNA EXTRACTION:, A high concentration of chaotropic reagent, NaI, and an anionic detergent participate in solubilization of the proteins and lipids contained in biological samples. After addition of isopropanol to the mixture, nucleic acids are co-precipitated with polysaccharide glycogen as a carrier, while other components remain soluble in the solution phase., Ref.:, Ishizawa, M., Kobayashi, T. and Matsuura, S., ''Simple procedure of DNA Isolation from Human Serum'',, Nucleic Acids Res., ,, 19, , 5792 (1991)., Hui Cai, Xuelin Gu, Mary S. Scanlan, and ChrisR. Lively,: Development of a quantitative PCR assay for residual mouse DNA and comparison of four sample purification methods for DNA isolation., Journal of Pharmaceutical and Biomedical Analysis, 55, 71-77 (2011)., This method was listed on USP39-NF34, page 1413/Pharmacopeial Forum. Vol.41(4).