This product is for research use only. Do not administer it to human.DNA extraction from serum and plasma, DNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and plasma. Based on the Sodium Iodide (NaI) method, 1, , this kit enables the whole procedures done in a single micro-centrifuge tube without using hazardous phenol/chloroform.Recently, tumor-specific genes have been amplified and detected in the serum and plasma of patients with various diseases such as lung, breast and colon cancer. Many of these reference articles are currently being published, 2,3,4, .The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield., Features, High DNA yield (about 100%) from small amount of serum and plasma, The DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA., Safe operation No phenol or chloroform required., Minimum contamination: The entire extraction of DNA can be done in a single centrifuge tube, Complete removal of lipid derived from blood, Less variability in extraction from sample to sample, Kit Contents, (50 reactions), Enzyme reaction solution 1 x 10 mL, Protein digestion solution 1 x 250 uL (uL: micro-L), Sodium iodide solution 1 x 15 mL, Alcohol solution 1 x 30 mL, Washing Solution (A) 1 x 50 mL, Washing Solution (B) 1 x 50 mL, References, Ishizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.:, Nucleic Acids Res., ,, 22, , 1774 (1994)., Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.:, Clin. Cancer Res., ,, 5, , 2689 (1999), Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva M. J., Navarro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.:, Cancer Res., ,, 59, , 3251 (1999), Shao, Z. M., Wu, J., Shen Z. Z. and Nguyen, M.:, Clin. Cancer Res., ,, 7, , 2222 (2001), 1.High DNA yield from small amount of serum and plasmaThe DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA than in solid-phase2. Safety of operation - Hazardous phenol and chloroform are not employed as reagents3. Minimizing the cross and extraneous contaminationThe entire extraction of DNA is done in a single centrifuge tube.4. Complete removal of lipids derived from blood using a specialized alcohol solution.5. Extraction of high quality DNA without PCR inhibition
