Wako, 302-14686, BAMBANKER (TM)

Serum-Free Cell Freezing Medium, BAMBANKER (TM) is a serum-free medium for long-term freezing at -80 degrees C and preservation of valuable culture cells such as tumor cells and normal cells., Features, Ready-to-use medium for preservation of cells,, Usable without diluting,, No program freezer is required,, Rapid and long-term freezing and preservation in a deep freezer (-80C),, Serum-free, Operating Procedure, 1) Centrifuge the cells in the logarithmic growth phase, and collect 5 x 10, 5, ~ 1 x 10, 7, cells., 2) Suspend the collected cells in 1 mL of this medium, and place the cells in cryotubes for freezing and preservation. Then, freeze and preserve the cells at -80C without preliminary freezing. The frozen cells at -80C can be preserved subsequently in liquid nitrogen., 3) Thawing must be carried out quickly in a constant-temperature chamber or bath., Note :The cells must be frozen in the logarithmic growth phase. :Certificate of analysis will be issued upon request., Sterility test, Endotoxin: Colorimetric method, Mycoplasma: Culture method, Fungi and bacteria: membrane filtration method, Applications, P3U1 (mouse myeloma cell line), K562 (human leukemia cell line), human gastric epithelial cells, human Gamma delta T cells, Daudi (human B cell line), PC12 (rat-derived adrenal pheochromocytoma), human B cell line, OKT4 (mouse hybridoma), monkey B cell line, Activated lymphocyte derived from human peripheral blood, Activated lymphocyte derived from mouse spleen, Manufactured by GC LYMPHOTEC Inc. (Japan).