Description
Serum-Free Cell Freezing Medium, BAMBANKER is a serum-free medium for long-term freezing at -80 degrees C and preservation of valuable culture cells such as tumor cells and normal cells, Features, Ready-to-use medium for preservation of cells, Usable without diluting, No program freezer is required, Rapid and long-term freezing and preservation in a deep freezer (-80 C), Serum-free, Operating Procedure, Centrifuge the cells in logarithmic growth phase*, and collect 5 x 10^5 ~ 1 x 10^7 cells., Suspend the collected cells in 1 mL of this medium, and place the cells in cryotubes for freezing and preservation. Then, freeze and preserve the cells at -80 C without preliminary freezing. The cells frozen at -80 C can be preserved subsequently in liquid nitrogen., Thawing must be carried out quickly in a constant-temperature chamber or a bath., Sterility Test, **, Endotoxin: chromogenic substrate method, Mycoplasma: Fluorescent antibody method, Fungi and bacteria: As per Japanese Pharmacopoeia, Note* : The cells must be frozen in logarithmic growth phase.**: Certificate of analysis will be issued upon request., Applications, [Cells preserved well by cryopreservation test]:, P3U1 (mouse myeloma cell line), K562 (human leukemia cell line), human gastric epithelial cells,, etc., Daudi (human B cell line), PC12 (rat-derived adrenal pheochromocytoma), human B cell line, OKT4 (mouse hybridoma), monkey B cell line, Activated lymphocyte derived from human peripheral blood, Activated lymphocyte derived from mouse spleen, Manufactured by LYMPHOTEC Inc. (Japan).
