Description
This product is for research use only. Do not administer it to human., for Recovery of nucleic acids from agarose gels, β-agarase is an enzyme which hydrolyzes β-1,4 linkages in agarose to produce neoagaro- oligosaccharides. Agarose digested by β-agarase does not gelate again, therefore, nucleic acids can be recovered from agarose gels., Thermostable β-Agarase, has a higher thermostability and stronger hydrolyzing activity than conventional β-agarase. Furthermore, a simple protocol allows for quick DNA and RNA purification. This enzyme is particularly suitable for purification of intact large DNA., This enzyme was isolated from deep-sea microorganism by Japan Agency for Marine-Earth Science and Technology (JAMSTEC) using the submergible SHINKAI 6500., Features:, Simple and short protocol (Reaction is completed in only 10 minutes)., Can be used on standard agarose as well as low-melting point agarose., Hydrolyzing gel solution is directly available for various applications. Such as cloning, restriction endonuclease digestion, sequencing,, etc, ., Can effectively extract intact large DNA from agarose gels., Procedures 1, Transfer 200 mg (200 µL) gel slice to a 1.5 mL microcentrifuge tube., Melt completely the gel slice by incubation for 5~10 minutes at peculiar melting temperature of each agarose., Transfer the tube to water bath (or heat block,, etc, .) set at 50~65 °C., Add 6 units of the, Thermostable β-Agarase, ., Incubate at 50~65 °C for 5~10 minutes to digest agarose. During incubation, tap the tube every 3~5 minutes., Chill on ice for 5 minutes, and make sure that agarose does not gelate again. The agarase-digested sample c
