Wako, 315-02504, ISOGEN

This product is for research use only. Do not administer it to human., ISOGEN and ISOGEN-LS both employ an extraction procedure for total RNA purification from a wide range of samples of human, animal, plant and bacterial origin. ISOGEN proves useful for samples with small quantity availability, because it provides a high relative yield and high purity of intact RNA., Meanwhile, time involvement is minimal, as a somewhat laborious CsCl gradient need not be incorporated, attributable to this ISOGEN procedure., ISOGEN is suitable for samples such as tissues and culture cells., ISOGEN-LS is suitable for high volume samples, examples of which include blood and fluid samples which are difficult to concentrate., Characteristics:, [1] ISOGEN and ISOGEN-LS are homogenous solutions, consisting mainly of phenol and guanidinium thiocyanate., [2] The extraction procedures which are employed here, induce a liquid phase separation, resulting in a conveniently tinted aqueous RNA layer. [ISOGEN: blue, ISOGEN-LS: red], [3] Through employment of the provided protocol, RNA, DNA, and protein are separated from one another in one series of steps., [4] The ISOGEN procedures are easy and quick. Each single separation provides, -RNA in approximately 1 hour,, -DNA in a little over 2 hours,, -and protein extraction in a little over 2 and a half hours., [5] High Recovery Rate