Waters, 186002180, BioSuite DEAE Anion-exchange Column 1000Å 10 µm, 7.5mm x 75 mm, 1/pk

Waters BioSuite DEAE Anion-Exchange HPLC Column separates biomolecules based on differences in surface charge using diethylaminoethyl anion-exchange functionality. This porous chromatography format employs 10 µm polymer particles with 1000 Å pores in a 7.5 mm internal-diameter by 75 mm long column. The large pore structure is suited to the movement of proteins and other relatively large biomolecules through the stationary phase. DEAE provides a weak anion-exchange mode that can be tuned through mobile-phase pH and ionic strength. Salt gradients may be used to resolve bound species through differences in charge, while changes in pH can alter both protein charge and ligand interaction. The product supports ion-exchange HPLC method development rather than hydrophobic reversed-phase separation. The specified stationary-phase pH compatibility spans approximately 2 to 12. Use filtered buffers and control sample precipitation when introducing high-salt eluents. Confirm that the HPLC pump, fittings and detector are compatible with the selected buffers. The product is one packed analytical ion-exchange column, not loose resin or a dialysis device.