| Chemistry | Amide |
|---|---|
| Separation Mode | Hydrophilic Interaction (HILIC) |
| Particle Substrate | Hybrid |
| pH Range Min | 2 pH |
| pH Range Max | 11 pH |
| Maximum Pressure | 6000 psi (415 Bar) |
| Endcapped | No |
| Silanol Activity | Low |
| Particle Shape | Spherical |
| Particle Size | 3.5 µm |
| Endfitting Type | Waters |
| Pore Size | 130 Å |
| Format | Column |
| Surface Area | 185 |
| System | HPLC |
| Particle Technology | BEH |
| USP Classification | L68 |
| Inner Diameter | 2.1 mm |
| Length | 150 mm |
| Carbon Load | 12 % |
| UNSPSC | 41115709 |
| Brand | XBridge |
| Product Type | Columns |
| Units per Package | 1 pk |
Answer:It uses hydrophilic-interaction liquid chromatography (HILIC) to retain polar analytes such as carbohydrates and sugars.
Answer:It contains 3.5 µm particles with 130 Å pores in a 2.1 × 150 mm column.
Answer:The XBridge BEH Amide column supports a pH range of 2–11 within its pressure and method limitations.
The Waters XBridge BEH Amide column is designed for hydrophilic-interaction liquid chromatography of highly polar compounds. Its bridged ethyl hybrid (BEH) particles carry an amide-bonded stationary phase, providing retention mechanisms suited to carbohydrates, sugars, and other analytes that are weakly retained on conventional reversed-phase C18 columns. The column measures 2.1 mm internal diameter by 150 mm length and contains 3.5 µm particles with 130 Å pores. The chemistry supports a broad specified pH operating range of 2–11 and a maximum pressure of 6000 psi (415 bar). Mobile-phase composition, water content, and equilibration are especially important for repeatable HILIC retention. Aqueous-rich injection solvents may alter peak focusing for strongly retained polar compounds, so sample solvent selection should be matched to the method. The format is supplied as one analytical HPLC column.
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