Waters, 186005291, XSelect CSH C18 Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1/pk

The Waters XSelect CSH C18 HPLC column uses Charged Surface Hybrid particle technology to provide alternative selectivity to conventional silica-based C18 columns. The hybrid stationary phase is designed to improve chromatographic peak shape and sample loading for many basic compounds, which may interact strongly with residual surface sites on other phases. This analytical column contains 5 µm particles with nominal 130 Å pores and measures 4.6 × 250 mm. Its longer packed bed can provide increased resolving capacity for complex mixtures when appropriate flow rate and pressure are maintained. The column has a stated pH operating range of 1 to 11 and a 415 bar maximum pressure rating. Prepare mobile phases consistently, control buffer concentration and allow sufficient equilibration after solvent changes. Sample filtration and an optional guard strategy can protect the column from fouling. Compare method performance using efficiency, retention, selectivity and peak symmetry rather than assuming equivalence with other C18 packing materials.