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| Chemistry | C18 |
|---|---|
| Separation Mode | Reversed Phase |
| Particle Substrate | Hybrid |
| pH Range Min | 1 pH |
| pH Range Max | 12 pH |
| Maximum Pressure | 18000 psi (1240 Bar) |
| Endcapped | Yes |
| Silanol Activity | Low |
| Particle Shape | Spherical |
| Particle Size | 1.7 µm |
| Endfitting Type | Parker-style |
| Pore Size | 130 Å |
| Format | Premier Column |
| Surface Area | 185 |
| System | UPLC |
| Particle Technology | BEH |
| Technique | LC |
| USP Classification | L1 |
| Inner Diameter | 2.1 mm |
| Length | 100 mm |
| Carbon Load | 18 % |
| eCord | Yes |
| UNSPSC | 41115709 |
| Brand | ACQUITY |
| Product Type | Columns |
| Units per Package | 1 pk |
Answer:It reduces unwanted adsorption of metal-sensitive analytes to the column hardware, which can improve peak shape and recovery.
Answer:The column contains 1.7 µm BEH C18 particles with 130 Å pores in a 2.1 × 100 mm format.
Answer:The specified operating range is pH 1–12 and up to 18,000 psi (1,240 bar), subject to the limits of the full LC system.
Waters ACQUITY Premier BEH C18 columns combine sub-2 µm reversed-phase chromatography with MaxPeak High Performance Surface hardware. The 2.1 × 100 mm column contains 1.7 µm BEH hybrid particles with 130 Å pores and a trifunctionally bonded, endcapped C18 stationary phase. Its specially treated hardware reduces unwanted interactions between metal-sensitive analytes and exposed metal surfaces, supporting more consistent peak shape and recovery for susceptible compounds. The hybrid packing provides a specified pH range of 1–12, and the maximum column pressure is 18,000 psi (1,240 bar). Use a UPLC system capable of operating within the complete flow-path pressure limit and equipped with compatible low-dispersion connectors. Prepare clean, fully soluble samples, and maintain the mobile-phase composition required by the analytical method. Evaluate performance using analyte recovery, peak tailing, retention reproducibility, resolution, and pressure rather than assuming all compounds benefit equally from surface treatment.
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