Waters, PSS830914, Spherisorb Cyano (CN) Column, 80Å, 5 µm, 4.6 mm X 200 mm, 1/pk

Spherisorb Cyano is a silica-based HPLC column containing propyl-cyano bonded groups. This 80 Å, 5 µm packing is supplied in a 4.6 × 200 mm format for analytical normal-phase chromatography, where the polar cyano surface offers different selectivity from bare silica or alkyl-bonded reversed-phase media. The column is conditioned and shipped in normal-phase solvent, making solvent compatibility particularly important during initial installation and subsequent method changes. Polar analytes can show useful differences in adsorption and elution order as mobile-phase polarity is adjusted. Keep water content and solvent composition controlled when reproducibility is essential, and verify that all components remain miscible before switching solvents. Filter samples to reduce the risk of inlet blockage and maintain a consistent equilibration period after changing the eluent. The cyano phase is not interchangeable with a C18 column merely because both use silica substrates. A method transfer must account for the distinct retention mechanism and validated operating conditions.