Thermo Fisher
1000 ppm Standard Grade COD, Environmental Express
SKU · B1030
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This collection brings together Thermo Fisher buffer solutions and certified standards you rely on across cell culture, protein work, electrochemistry, chromatography, and mass spectrometry. Browse ready-to-use PBS/Tris/HEPES, pH and conductivity standards, LC/IC reference mixes, and LC-MS calibration solutions. As a distributor, Iright can supply a wide range of Thermo Fisher brands (Gibco, Thermo Scientific Chemicals, Orion, Pierce, Dionex) and provide quick COA/SDS on request.
Stable pH and ionic strength are the backbone of reproducible methods. In this section, you’ll quickly pinpoint the right buffer family and grade—cell-culture PBS, molecular-biology Tris-HCl, or zwitterionic HEPES—then decide on concentration, sterility, and packaging that fit your workflow and documentation needs.
At-a-glance buffer families
| Buffer system | Typical pH window | Common concentrations | Notes / where it fits |
|---|---|---|---|
| PBS (w/ or w/o Ca/Mg) | ~7.2–7.4 | 1×, 10× | Rinsing cells, reagent prep; cell-culture options are sterile-filtered and free of Ca/Mg to avoid unwanted adhesion. |
| Tris-HCl | ~7.5–8.8 | 0.5 M, 1 M | Molecular biology grade stocks for nucleic-acid/protein work; low-endotoxin choices available. |
| HEPES | ~7.0–8.5 | 1 M | Zwitterionic buffer with strong capacity near neutral pH; widely used in cell culture. |
Quantitation and daily QC depend on traceable references. Below you’ll find electrochemistry standards for pH and conductivity, IC multi-anion standards for retention/response checks, and LC-MS calibration solutions spanning positive/negative modes and extended mass ranges.
What you’ll use and when
Starting from the job at hand speeds selection. Use the matrix below to match your task to the right product type and constraints (sterility, volatility for MS, traceability for audits).
| Application | Recommended type | Key parameters to confirm | Why it fits |
|---|---|---|---|
| Rinsing and transporting cells | PBS (no Ca/Mg), sterile-filtered | pH 7.4, 1×, sterile, COA/SDS | Minimizes unwanted adhesion; ready to use in tissue culture. |
| Nucleic-acid/protein buffer prep | Tris-HCl stock | 1 M or 0.5 M; low-endotoxin/MolBio grade | Clean stock simplifies downstream prep and documentation. |
| Cell-friendly buffering near neutral pH | HEPES stock | 1 M; desired target pH (7.0–7.5–8.5) | Strong capacity around physiological conditions. |
| Calibrating pH meter | pH buffer solutions | 4.01/7.00/10.01 (± tolerance), NIST-traceable | Brackets routine measurements with traceability. |
| Calibrating conductivity/TDS | Conductivity standards | 100 µS, 1413 µS, 12.9 mS, 111.9 mS | Covers low to high ionic strength ranges. |
| IC routine checks | Dionex combined anion standard | Bottle size; target species & mg/L | One bottle validates retention and response. |
| LC-MS mass accuracy | Pierce calibration solutions | Instrument family; positive/negative; extended range | Ready-to-use PTFE-bottled mixes for fast calibration. |
The list below highlights commonly requested items across buffer and standard categories so you can compare spec, volume, and brand line at a glance.
| Product (brand line) | Type | Grade / Traceability | Spec | Volume | Sterile | Catalog No. |
|---|---|---|---|---|---|---|
| PBS, pH 7.4 (Gibco) | Buffer | Cell-culture grade | 1×, pH 7.4; no Ca/Mg, no phenol red | 500 mL | Yes | 10010023 |
| PBS 1×, sterile-filtered (Thermo Scientific Chemicals) | Buffer | Chemicals grade | 1× PBS | 500 mL | Yes | J61196.AP |
| Tris-HCl 1 M, pH 8.0 (Thermo Scientific Chemicals) | Buffer stock | MolBio grade, ultrapure | 1 M, pH 8.0 | 100 mL | — | J22638.AE |
| Tris-HCl 0.5 M, pH 8.0, low endotoxin | Buffer stock | Low endotoxin | 0.5 M, pH 8.0 | 250 mL | — | J67510.AK |
| HEPES 1 M, pH 7.5 (Thermo Scientific Chemicals) | Buffer stock | Chemicals grade | 1 M, pH 7.5 | 500 mL | — | J60712.AP |
| HEPES 1 M (Gibco) | Buffer stock | Cell-culture use | 1 M | 20 mL | — | 15630106 |
| Orion pH Buffer 4.01 | pH standard | NIST-traceable | 4.01 ± 0.01 | 475 mL | — | 910104 |
| Orion pH Buffer 7.00 | pH standard | NIST-traceable | 7.00 ± 0.01 | 475 mL | — | 910107 |
| Orion Conductivity Standard | Conductivity standard | Traceable | 1413 µS/cm, bottle | — | — | 011007 |
| Orion Conductivity Standard | Conductivity standard | Traceable | 12.9 mS/cm, bottle | — | — | 011006 |
| Pierce LTQ ESI Positive Ion Calibration Solution | LC-MS calibration | Mass-spec grade | Positive mode | 10 mL | — | 88322 |
| Pierce Triple Quadrupole Calibration Solution | LC-MS calibration | Mass-spec grade | Positive mode (TSQ) | 10 mL | — | 88325 |
| Pierce Triple Quad Calibration, Extended Mass Range | LC-MS calibration | Mass-spec grade | Extended range | 10 mL | — | 88340 |
| Dionex Combined Seven Anion Standard II | IC standard | NIST-traceable | Multi-anion mix | 100 mL | — | 057590 |
Getting storage right protects stability and your results. Most ready-to-use buffers ship at ambient and store at room temperature or 2–8 °C; avoid contamination by aliquoting, using clean tips, and capping promptly.
For LC-MS work, prefer volatile salts/buffers (e.g., ammonium formate/acetate) in the method itself and keep non-volatile buffers for offline steps. (For PBS cell-culture packs, check sterile-filtered status and Ca/Mg content in the spec table or COA.)
Quick reminders
Daily routines are short and repeatable. Use these quick patterns to map products to tasks and keep your documentation clean.
A brief set of answers helps you move from shortlist to purchase without detours. If you need a formal quotation or documentation packet, contact Iright anytime.
Q1. Do I need Ca/Mg in PBS for cell handling?
Generally no—choose PBS without Ca/Mg for washing and transfers to avoid unwanted adhesion; confirm sterile-filtered status for direct use in culture.
Q2. Which pH buffers should I keep on hand?
Most labs maintain 4.01, 7.00, and 10.01 buffers; alkaline labs may add 12.46. Pick NIST-traceable bottles or 5×60 mL packs for field use.
Q3. What conductivity values cover routine checks?
100 µS/cm, 1413 µS/cm, 12.9 mS/cm, and 111.9 mS/cm span low to high ionic strengths for most meters and methods.
Q4. How do I choose between LTQ/Triple Quad/FlexMix LC-MS solutions?
Match the solution to your instrument family and mode: LTQ positive, Triple Quad (TSQ), or FlexMix for positive/negative with extended ranges on Orbitrap platforms.
Q5. Are IC standards traceable?
Dionex combined anion standards are designed for routine determinations and supplied with COA; select size and formulation that match your method.
Tell us the catalog numbers, volumes, and any certificate requirements (e.g., NIST traceability, endotoxin limits). Iright will confirm stock, lead time, and documentation, and can help you standardize a short, auditable list of buffers and standards for your lab. (If you prefer equivalents under the same brand line, we can suggest them side by side.)
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