Thermo Fisher
5X Kinase Buffer A, 100 ml
SKU · PV6135
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From routine PCR to delicate RNA prep and phospho-signaling studies, you need enzymes that catalyze clean reactions—and inhibitors that safeguard samples. This collection organizes Thermo Fisher solutions by task so you can choose fast. As an authorized distributor, Iright supplies a broad Thermo Fisher range with quote-ready availability.
Enzymes here drive core bench reactions—cutting, copying, joining, or trimming nucleic acids—while inhibitors block unwanted activities that degrade your results. Think high-fidelity polymerases for accurate amplification, FastDigest restriction enzymes for rapid digests, and RNase/protease/phosphatase inhibitor cocktails to protect fragile RNA and post-translational modifications.
To shorten selection time, start with the family that maps to your workflow. Scan the brief overview for fit, then jump to representative SKUs you can add to carts or RFQs without leaving the page.
PCR & RT Polymerases — Choose high-fidelity chemistry for cloning and variants, or hot-start options to suppress non-specific amplification. Representative picks include Thermo Scientific Phusion High-Fidelity DNA Polymerase (Cat. F530S, F530L) and Invitrogen Platinum SuperFi II DNA Polymerase (Cat. 12361010, 12361050).
Restriction Enzymes (FastDigest series) — Rapid digests in 5–15 minutes with a universal buffer that streamlines single or double digests, minimizing buffer swaps and star activity risk. Example: FastDigest XbaI (Cat. FD0684, FD0685).
DNA Ligation — Robust ligation for cohesive/blunt ends and nick repair. Popular options include Thermo Scientific T4 DNA Ligase (Cat. EL0011, EL0014).
RNA Protection — Keep RNases A/B/C at bay during extraction and RT. RiboLock RNase Inhibitor (40 U/µL) offers convenient pack sizes (Cat. EO0381, EO0382, EO0384).
Protein Protection — Preserve proteins and phosphorylation states right from lysis with Halt Protease & Phosphatase Inhibitor Cocktails, including EDTA-free variants for metal-dependent assays (Cat. 78440, 78442, 78443).
| Catalog # | Product name | Family | Pack/Units | Typical use |
|---|---|---|---|---|
| F530S | Phusion™ High-Fidelity DNA Polymerase (2 U/µL) | PCR (Hi-Fi) | 100 rxns | Accurate cloning, GC targets |
| 12361010 | Platinum™ SuperFi II DNA Polymerase | PCR (Hi-Fi) | 100 rxns | Universal 60 °C annealing, tough templates |
| FD0684 | FastDigest XbaI | Restriction | 300 rxns | 5–15 min digests, universal buffer |
| EL0011 | T4 DNA Ligase (5 U/µL) | Ligation | 1,000 U | Blunt/cohesive ligation, nick repair |
| EO0381 | RiboLock RNase Inhibitor | RNA protection | 2,500 U | RT/qPCR, RNA extraction |
| 78440 | Halt Protease & Phosphatase Inhibitor Cocktail (100×) | Protein protection | 1 mL | PTM-sensitive lysis |
| 78443 | Halt Protease & Phosphatase Inhibitor Cocktail, EDTA-free (100×) | Protein protection | 24×100 µL | Metal-dependent enzymes |
When options look similar, anchor the choice to your bench goal and constraints. The matrix below pairs common tasks with recommended types and practical notes, so you can move from question to cart decisively.
| Task at the bench | Recommended type | Why it helps | Check before ordering |
|---|---|---|---|
| High-GC or long amplicons | High-fidelity polymerase (e.g., Phusion, SuperFi II) | Higher processivity/fidelity reduces drop-offs and errors | Template quality; annealing temp window (e.g., 60 °C universal) |
| Fast screening digests | FastDigest restriction enzyme(s) | 5–15 min single/dual digests; universal buffer simplifies setup | Recognition site methylation; downstream ligation compatibility |
| Blunt/cohesive cloning | T4 DNA Ligase | Efficient end-joining repairs nicks | ATP in buffer; insert: vector ratios; temperature window |
| RNA extraction/RT | RNase inhibitor (RiboLock) | Shields RNA from RNases A/B/C | Final concentration in mix; detergent compatibility |
| Preserve phospho-signaling | Protease + phosphatase inhibitor cocktail (Halt) | Limits proteolysis and dephosphorylation during lysis | EDTA-free if using metal-dependent enzymes; lysis buffer recipe |
Buffers determine speed, star activity risk, and downstream success. FastDigest enzymes share a universal buffer so you can combine two enzymes without changing buffers and finish in minutes, which also improves ligation hand-off. For lysis, EDTA-free inhibitor cocktails avoid chelating metal cofactors in assays or phosphatases you may need later.
Match performance to the decision you’re making with the data. High-fidelity polymerases like Platinum SuperFi II and Phusion reduce errors for cloning and variant calling; hot-start formulations curb non-specific amplification when reaction setup or GC content is challenging. Universal-annealing buffers simplify primer design at 60 °C for bread-and-butter PCR.
Shipping and storage protect activity as much as formulation does. Follow the datasheet for temperature and stability; inhibitor cocktails such as Halt are stored at 4 °C, while many enzymes ship cold and are kept frozen to minimize activity loss. Aliquot where practical to reduce freeze–thaw cycles and keep buffers capped tightly.
These short, copy-pastable recipes cover common setups and help new hands match the datasheet in one pass. Adjust volumes for your tube size and confirm product-specific notes on the product page before first use.
High-GC PCR (25 µL, SuperFi II/Phusion)
Mix polymerase master mix per datasheet; add 0.5 µM primers, 200 µM dNTPs, 1–10 ng gDNA/cDNA.
Initial denaturation as recommended; cycle 98 °C denaturation, 60 °C universal annealing or Tm-based, and 72 °C extension (15–30 s/kb).
Finish with 72 °C 5 min; hold 4 °C; analyze on agarose.
Fast double digest → ligation (FastDigest + T4 Ligase)
Digest 0.5–1 µg plasmid/insert with two FastDigest enzymes in universal buffer, 37 °C for 10–30 min.
Heat-inactivate or purify if required; set ligation with T4 DNA Ligase at recommended ratios and temperature.
Transform; plate and screen.
Using RNase inhibitor (RiboLock)
Add per datasheet final U/µL to extraction or RT reactions.
Keep solutions RNase-free; avoid repeated warm-cold cycles.
Proceed to cDNA synthesis or downstream PCR.
Adding protease/phosphatase inhibitor cocktails (Halt)
Spike lysis buffer to 1× just before use (EDTA-free for metal-dependent assays).
Keep samples cold; shorten time to clarification.
Proceed to BCA, Western, or MS prep per protocol.
Most purchase delays come from the same questions. Use these quick answers to move forward, and check the product page when you need line-by-line specs.
Do I really need high-fidelity for routine PCR?
If you’ll clone or sequence the amplicon, yes—error rates compound downstream. Use SuperFi II or Phusion; for screening only, a standard Taq may suffice.
Can FastDigest enzymes really do a double digest without buffer swaps?
Yes—FastDigest shares a universal buffer designed for single or combined digests in 5–15 min; verify methylation sensitivity for your site.
When should I choose EDTA-free inhibitor cocktails?
Pick EDTA-free when assays depend on metal ions (e.g., metalloproteases, kinase assays). Otherwise, standard cocktails offer broad protection.
Why is my ligation poor with blunt ends?
Increase insert:vector ratio, check ATP in the ligase buffer, and confirm temperature/time. If necessary, polish ends or use cohesive-end strategies.
Are RNase inhibitors compatible with detergents?
Most workflows are compatible at recommended final concentrations; confirm product notes if you use harsh surfactants.
Tell us what you’re building—screening clones, protecting RNA, or preserving phospho-signals—and we’ll match stock to your protocol. Iright can supply Thermo Fisher polymerases, FastDigest enzymes, ligases, and inhibitor cocktails across pack sizes, with documents ready for your purchase notes.
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