Thermo Fisher
1000μL Polypropylene 96-Well Deep Well PCR Plates
SKU · 0030505.204
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Thermo Fisher microplates bring together reliable surfaces, clear optical paths, and SBS-standard footprints so you can run ELISA, fluorescence/luminescence assays, cell imaging, HTS screens, and routine storage with confidence. On this Collection page you’ll find application-driven guidance, surface and format comparisons, and representative SKUs verified against Thermo Fisher’s catalog. As an authorized distributor, Iright can source a broad range of Thermo Fisher (Nunc™/Thermo Scientific™) plates and accessories to match your workflow.
Choosing starts from the assay. Plate color and surface chemistry directly affect signal, background, and binding performance; well geometry and material affect volume recovery and automation. Use the quick map below to align each application with the most suitable plate style before you drill into surfaces and formats.
ELISA / Solid-phase assays → Clear polystyrene immuno plates with high-binding surfaces (e.g., MaxiSorp™) for protein adsorption and consistent colorimetric reads.
Fluorescence → Black plates minimize crosstalk and autofluorescence; optical-bottom variants support cell-based imaging.
Luminescence → White plates maximize reflectance for higher signal; optical bottoms support microscopy when needed.
Cell culture & imaging → Nunclon™ Delta TC-treated surfaces improve adhesion; Edge 2.0 designs reduce edge effects during long incubations.
Compound/DNA storage & sample processing → Polypropylene (PP) low-binding plates (including DeepWell™) offer solvent resistance and complete recovery.
Surfaces that look alike can behave very differently. High-binding immuno surfaces adsorb proteins for ELISA; hydrophilic TC-treated surfaces favor adherent cells; PP “low-binding” storages minimize biomolecule loss. The table clarifies practical fit so you pick once and run with fewer repeats.
| Surface type | Typical use | Binding/interaction | Notes |
|---|---|---|---|
| MaxiSorp™ | Protein ELISA/antibody capture | Energy-treated high protein binding | Classic clear/black immuno plates; legacy SKUs exist; see current immuno plate families. |
| PolySorp™ | Hydrophobic ligands/antigens | Hydrophobic binding | Often used when protein adsorption needs are lower or more selective. |
| Nunclon™ Delta (TC-treated) | Adherent cell culture, imaging | Hydrophilic, promotes adhesion | Offered across 96/384-well and Edge 2.0 formats to reduce edge effects. |
| Low-binding PP | Storage/processing of DNA, proteins, and solvents | Minimizes adsorption | Polypropylene plates tolerate solvents; shared-wall DeepWell variants increase well diameter for mixing. |
Selecting a format balances throughput, optics, and instrument compatibility. Standard 96-well suits most ELISAs; 384/1536 drive HTS density; black/white/clear decide detection physics; and optical polymer bottoms enable microscopy without leaving microplate workflows. All plates conform to SBS/ANSI footprints for automation and readers.
Colors: Clear (absorbance/ELISA), Black (fluorescence), White (luminescence).
Bottoms: Flat/U/V; optical polymer bottom or coverglass base for imaging and sensitive reads.
Materials: Polystyrene for assays/cell work; polypropylene for storage/solvents.
This short list highlights representative, widely used SKUs to anchor your selection. Each entry links to a distinct use case—immuno binding, optical imaging, TC culture, or low-binding storage—so you can map directly to your assay and platform. (More sizes/packaging exist under each family.)
| Product (family) | Catalog No. | Color / Bottom | Surface | Wells | Typical use |
|---|---|---|---|---|---|
| Clear Flat-Bottom Immuno 96-Well Plates | 442404 | Clear/flat | Immuno (family options) | 96 | General ELISA & colorimetric assays. |
| Clear Flat-Bottom Immuno 96-Well, PolySorp | 475094 | Clear/flat | PolySorp | 96 | Hydrophobic binding ELISAs/ligands. |
| Black 96-Well Immuno Plates, MaxiSorp | 437111 | Black/flat | MaxiSorp | 96 | Low-background fluorescence ELISAs. |
| 96-Well Black Plate (non-treated) | 237108 | Black/flat | Non-treated PS | 96 | Fluorescence assays; robotics-ready. |
| 96-Well White Plate (non-treated) | 236108 | White/flat | Non-treated PS | 96 | Luminescence assays; high reflectance. |
| Optical-Bottom 96-Well, Black, TC | 165305 | Black/optical | Nunclon Delta (TC) | 96 | Cell-based imaging & FI/LI reads. |
| Optical-Bottom 96-Well, White, TC | 165306 | White/optical | Nunclon Delta (TC) | 96 | Imaging with luminescent detection. |
| MicroWell 96-Well, Nunclon Delta, with lid | 167008 | Clear/flat | TC-treated | 96 | Routine adherent culture & assays. |
| PP 96-Well Sample Processing & Storage | 249944 | Natural / V-bottom | Polypropylene (low-binding) | 96 | DNA/protein storage & solvent use. |
| DeepWell™ PP 96-Well (shared-wall) | 95040452 | Natural/deep | Polypropylene | 96 | High-capacity storage & mixing. |
| Edge 2.0 96-Well, Non-treated | 267427 | Clear/flat | Non-treated | 96 | Reduces edge evaporation in long incubations. |
| Edge 2.0 96-Well, TC-treated | 167425 | Clear/flat | Nunclon Delta (TC) | 96 | Even viability across the plate; incubations. |
Good plates need good closures. The right lid or film controls evaporation, preserves sterility, and protects optics. Choose sterile lids for culture and optical films for qPCR/imaging; use breathable membranes for gas exchange during growth or long incubations.
Microplate lids (sterile/non-sterile, 96/384): 250002 (sterile), 250003 (non-sterile), 264623 (96-well sterile, with condensation rings), 264611/264612 (384-well).
Adhesive/optical films: AB0558 (adhesive PCR seals), 4311971 (MicroAmp™ optical adhesive film for qPCR).
Breathable/vented seals: Nunc™ seals & foils range (e.g., 241205 rayon breathable; 5701TS1 polyester).
Automation demands consistent geometry, stiffness, and readout windows. Nunc microplates—including Edge 2.0—follow ANSI/SBS standards and are compatible with robotic liquid handlers, stackers, and Thermo plate readers (absorbance, FI, LI, TRF). Optical-bottom designs pair with imaging systems without sacrificing throughput.
Implementation tips (brief):
Prefer skirted/rigid plates for pin tools and stackers; confirm barcode location for traceability.
Use black optical-bottom for fluorescence imaging; white optical-bottom for luminescence microscopy.
For long incubations, switch to Edge 2.0 to minimize edge effect, enabling all 96 wells (↑ throughput).
Q1. Black vs. white vs. clear—when to use each?
Use clear for absorbance/colorimetric ELISAs, black for fluorescence to reduce crosstalk, and white for luminescence to boost signal via reflectance. Optical bottoms support microscopy in black/white plates.
Q2. MaxiSorp vs. PolySorp—what’s the difference?
MaxiSorp is an energy-treated high-protein-binding surface for ELISAs; PolySorp is more hydrophobic for ligands or when lower binding is desired. Both are part of Thermo’s immuno plate families.
Q3. How do I reduce edge effects in cell-based assays?
Use Nunc Edge 2.0 plates; fill the outer moat with sterile water or media to buffer evaporation and improve viability uniformity across wells.
Q4. Which plates are for long-term or solvent exposure?
Choose polypropylene (PP) storage or DeepWell plates for solvent resistance and low adsorption of DNA/proteins; combine with compatible sealing films.
Q5. Are these plates automation-ready?
Yes—Nunc microplates follow ANSI/SBS standards and are validated across Thermo readers and common liquid handlers; confirm specific stacker tolerances and barcodes when ordering.
Tell us your assay type, readout (Abs/FI/LI/TRF), well density, surface, and whether you need optical bottoms or Edge 2.0. Iright will match SKUs (e.g., 165305/165306 for imaging, 437111 for MaxiSorp ELISA, 249944/95040452 for storage) and propose lead-time/packaging options, plus lids/seals to fit your incubators and readers.
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