Thermo Fisher
100X Pretreatment Solution
SKU · QVT0500
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This collection brings together Thermo Fisher’s core molecular biology reagents and kits used from sample to result—DNA/RNA extraction, cDNA synthesis, PCR/qPCR, cloning, electrophoresis, and basic analysis. As a distributor, Iright supplies a broad range of Thermo Fisher SKUs with documentation and technical support so you can select confidently and move faster from planning to purchase.
Across routine research and method development, you rely on dependable chemistries that perform consistently on your instruments and samples. This page organizes high-use Thermo Fisher lines by workflow so you can jump to what you need, compare essentials, and download the right documents for internal review.
Nucleic acid extraction & purification (column- and magnetic bead–based; automation-friendly options)
cDNA synthesis & RT-PCR (SuperScript™ IV systems)
PCR & qPCR master mixes (SYBR™ and probe/TaqMan™ chemistries; high-fidelity and hot-start enzymes)
Cloning & assembly (TOPO™ TA, GeneArt™ Gibson Assembly®; competent cells)
Electrophoresis & visualization (agarose tools, ladders, SYBR™ Safe stain)
Quantification & QC (Qubit™ dsDNA assays)
Start with your sample type and downstream readout, then match the chemistry. Consider sensitivity, inhibitor load, and whether you’ll automate. Use probe-based qPCR for maximal specificity, high-fidelity polymerases before cloning or sequencing, and magnetic-bead extraction when you need throughput and reproducibility.
Quick matches (goal → suggested solution):
Viral RNA/DNA from swabs or VTM → MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit (automation-ready, qPCR/NGS compatible).
Low-copy targets & inhibitor-rich RNA → SuperScript™ IV First-Strand Synthesis System (high inhibitor tolerance) + probe-based qPCR master mix.
Precise amplification for cloning/NGS → Platinum™ SuperFi™ II or Phusion™ high-fidelity polymerase.
Screening vs. confirmatory qPCR → SYBR™ Green master mix for fast screens; TaqMan™ Fast Advanced Master Mix for higher specificity and multiplexing.
Seamless multi-fragment cloning → GeneArt™ Gibson Assembly® HiFi kits or master mix; use TOPO™ TA for quick PCR-product cloning.
The items below are representative of popular choices in each step. Unless Thermo Fisher explicitly designates otherwise, treat these as RUO. Use the links in your internal checklist to pull datasheets, IFUs, and SDS for approval.
| SKU | Product name | Stage / use | Key specs (concise) | Format / pack | Notes |
|---|---|---|---|---|---|
| A42352 | MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit | Extraction | Magnetic-bead workflow for RNA/DNA from swabs, blood, VTM | Up to 100 preps | KingFisher-compatible; qPCR/NGS-ready. |
| 18091050 | SuperScript™ IV First-Strand Synthesis System | cDNA | High processivity; tolerant to inhibitors; with/without ezDNase options | 50 reactions | For RT-PCR/RT-qPCR from total or poly(A)+ RNA. |
| 12361010 | Platinum™ SuperFi™ II DNA Polymerase | PCR (Hi-Fi) | Proofreading; universal 60 °C annealing; blunt-end products | 100 reactions | For GC-rich/complex templates; cloning-grade PCR. |
| F530S | Phusion™ High-Fidelity DNA Polymerase (2 U/µL) | PCR (Hi-Fi) | HF & GC buffers; fast cycling; high fidelity | 100 units | Robust on GC-rich templates. |
| A25741 | PowerUp™ SYBR™ Green Master Mix | qPCR (dye) | Universal 2X mix; melt-curve compatible | 1 mL (and other sizes) | For singleplex gene expression screens. |
| 4444557 | TaqMan™ Fast Advanced Master Mix | qPCR (probe) | Passive ROX; UNG/dUTP; fast cycling | 1 × 5 mL (other sizes) | Broad instrument support; duplex-friendly. |
| K4575J10 | TOPO™ TA Cloning™ Kit (with One Shot™ TOP10) | Cloning | 5-minute ligation-free TOPO cloning | 10 reactions (25/50 also) | For TA-ended PCR products. |
| A46626 | GeneArt™ Gibson Assembly® HiFi Cloning Kit (electrocompetent) | Assembly | One-step isothermal assembly; multi-insert | 10 reactions | Includes ElectroMAX™ DH10B cells. |
| Q32851 | Qubit™ dsDNA HS Assay Kit | Quantification | 0.1–120 ng range; dsDNA-selective | 100 assays | Pair with Qubit™ fluorometer. |
| 10787018 | 1 Kb Plus DNA Ladder | Electrophoresis | 100–15,000 bp sizing; reference band | 500 applications | Agarose-gel compatible. |
| S33102 | SYBR™ Safe DNA Gel Stain | Visualization | Less-hazardous EtBr alternative; blue-light/UV | 400 µL | Sensitive DNA staining. |
Small chemistry differences and passive reference requirements can affect results across platforms. Use this section to harmonize protocols across instruments and assays while minimizing re-optimization and reducing reruns in your lab schedule.
SYBR™ vs. TaqMan™: SYBR™ Green mixes are ideal for rapid screening and budget-sensitive workflows; probe-based TaqMan™ improves specificity and supports duplexing/multiplexing when targets or backgrounds are complex. Use melt curves for SYBR™ assays; design probes to avoid SNPs and indels in critical regions.
Passive reference/filters: Confirm ROX/passive reference handling in your instrument’s software; most Applied Biosystems™ systems are straightforward. When moving between platforms, validate Ct shifts and baseline calls with 2–3 control genes.
High-fidelity PCR tips: For cloning-grade amplicons, choose SuperFi™ II or Phusion™. Start with the recommended buffer (HF or GC), reduce cycle number, and verify the fragment size with a ladder before cleanup. For GC-rich targets, include DMSO per IFU.
These short scenarios show how the kits align to common goals. Adapt the parameters to your template quality, target abundance, and instrument settings, and keep a small validation loop before scaling.
Low-copy viral RNA detection by RT-qPCR — Extract swab/VTM with MagMAX Viral/Pathogen; synthesize cDNA using SuperScript IV with ezDNase; quantify with TaqMan Fast Advanced Master Mix on your real-time system. Include a negative extraction control and a human control gene for process checks.
Precision amplicon generation prior to cloning/NGS — Amplify with Platinum SuperFi II (or Phusion) using the manufacturer’s HF buffer; confirm on agarose with 1 Kb Plus DNA Ladder; clean up before ligation or Gibson assembly. If needed, add A-overhangs for TA cloning.
Seamless multi-fragment vector build — Assemble 2–6 inserts with GeneArt Gibson Assembly HiFi; transform ElectroMAX DH10B included in the kit; screen colonies by colony PCR and confirm by Sanger. Use high-fidelity amplicons and 20–40 bp overlaps for robust assembly.
Accurate DNA quantification and safe visualization — Quantify purified DNA with Qubit dsDNA HS to avoid over- or under-loading; stain gels with SYBR Safe for sensitive, less-hazardous detection under blue light or UV.
Before ordering, teams often ask about fidelity, chemistry choice, and RUO/IVD boundaries. Keep answers brief so they can be reused in internal notes or purchase requests.
When should I choose a high-fidelity polymerase?
Use SuperFi™ II or Phusion™ when you’ll clone or sequence the product or when templates are GC-rich/complex. You’ll get proofreading and lower error rates than standard Taq, reducing downstream failures.
SYBR™ vs. TaqMan™—which is “better”?
Neither universally; SYBR™ is fast and economical for single-target screens, while TaqMan™ offers higher specificity and cleaner multiplexing. If you see non-specific melt peaks with SYBR™, confirm primers or move to probe-based chemistry.
Are these kits RUO or IVD?
Most items listed above are research use only unless Thermo Fisher specifically labels an IVD variant on the product page. Always check the IFU/labeling in your quality system before clinical use. (See product pages cited above.)
Ordering chemistry should be straightforward and traceable. Use the quote button or send your list of SKUs; we’ll return availability, lead times, and the document pack (datasheet, IFU, SDS, and CoA where applicable). If you need substitutions or automation guidance, tell us your instrument and throughput and we’ll propose compatible alternatives.
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