Abcam, ab102379-500µg, Goat Anti-Guinea pig IgG H&L (HRP) preadsorbed

Name: Goat Anti-Guinea pig IgG H&L (HRP) preadsorbed
Brand: Abcam
SKU: ab102379-500µg

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining.

Catalog Number: ab102379-500µg

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Category

Fluorophore-Conjugated Secondary Antibodies

Pack Size500 µg

Description

Key facts
Host species:Goat,
Target species:Guinea pig,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Minimal cross-reactivity:Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human,
Pre-adsorbed:Yes,
Conjugation:HRP,
Applications:ELISA, WB, IHC-P, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal,
Isotype:IgG,
Specificity:By immunoelectrophoresis and ELISA this antibody reacts specifically with guinea pig IgG and with light chains common to other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase., Storage buffer-pH: 6.8 - 7.4Preservative: 0.05% CMIT/MIT based preservativeConstituents: 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

FAQs

Question: Which primary antibody species does this secondary antibody target?

Answer:It recognizes guinea pig IgG heavy and light chains rather than a specific cellular antigen.

Question: What is the function of the HRP conjugate?

Answer:Horseradish peroxidase produces a detectable signal with a compatible colorimetric or chemiluminescent substrate.

Question: Why is the secondary antibody preadsorbed?

Answer:Cross-adsorption reduces unwanted binding to immunoglobulins from several other species and helps limit background staining.

Application Note

Abcam Goat Anti-Guinea Pig IgG H&L-HRP Preadsorbed Secondary Antibody enables enzymatic detection of guinea pig primary antibodies in immunoassays. The polyclonal goat antibody recognizes guinea pig immunoglobulin heavy and light chains and carries horseradish peroxidase for colorimetric or chemiluminescent substrate reactions. Preadsorption against several other animal immunoglobulins reduces cross-species background when samples contain mixed antibody components. Suitable research applications include western blotting, ELISA, immunocytochemistry and paraffin-section immunohistochemistry. The 500 µg presentation provides liquid antibody for dilution into a validated assay buffer. Determine the working concentration from the detection platform, membrane or specimen type and expected antigen abundance rather than using a single dilution for every experiment. Block nonspecific binding sites and wash thoroughly between incubations to preserve signal specificity. Select the HRP substrate and development interval to maintain a useful dynamic range. This reagent detects guinea pig immunoglobulins and is not a primary antibody against a specific cellular antigen.

Ordering

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