Abcam, ab32522-100µL, Anti-Caspase-7 antibody [E22]

Anti-Caspase-7 Antibody [E22] is a rabbit monoclonal antibody for studying the executioner caspase-7 in human cell and tissue samples. Caspase-7 is produced as an inactive precursor and can be processed to active fragments during apoptosis. Clone E22 recognizes both the pro-form and a p20 cleaved form, allowing changes in protein processing to be assessed in Western blot experiments. The antibody is also suited to paraffin-section immunohistochemistry, immunofluorescence, immunoprecipitation and intracellular flow cytometry in validated human material. Select the assay format to match the experimental question: Western blot can resolve precursor and cleavage-related bands, while imaging provides cellular localization without reliably distinguishing protein forms by size. Include untreated controls and an apoptosis-induction condition when evaluating activation. Apply suitable fixation, permeabilization and antigen retrieval protocols before staining. Caspase-7 signal should not be conflated with caspase-3 activity, and species outside validated human samples require independent evaluation. Use a consistent analysis window to compare conditions.