Description
Product details
What is this antibody validated in? Anti-Cdk6 antibody [EPR4515] (ab124821) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of Cdk6? Anti-Cdk6 [EPR4515] (ab124821) specifically detects a band for Cdk6 (UniProt: Q00534) at a molecular weight of 37kDa. Trusted by the scientific community Anti-Cdk6 [EPR4515] (ab124821) was first used in a scientific publication in 2012 and has been cited over 100 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Cdk6 antibody [EPR4515] (ab124821) has been confirmed by Western blot testing in CDK6 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR4515] also available for your convenience: ab124821, Alexa Fluor® 488 - ab198944, Alexa Fluor® 647 - ab198946, Carrier free - ab222395, PE - ab305713, APC - ab305714, HRP - ab305715, Alkaline Phosphatase - ab308887, Alexa Fluor® 594 - ab310613, Alexa Fluor® 555 - ab312141, Alexa Fluor® 568 - ab312627, Alexa Fluor® 750 - ab320982
Species reactivity Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Patented technology Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cdk6 functions as a regulator of the cell cycle. It participates in a complex with cyclin D to control the G1 phase progression. This activity is essential for the regulation of cell division and proliferation. The association with cyclin D allows Cdk6 to phosphorylate the retinoblastoma protein (Rb) leading to the release of E2F transcription factors that promote the expression of genes necessary for DNA synthesis.
Pathways
Cdk6 is an important component of the cell cycle control pathway. It interacts primarily with the retinoblastoma protein and Cyclin D1 in this context. The cell cycle pathway is critical for controlled cell proliferation and its dysregulation can lead to diseases like cancer. Another involved pathway is the PI3K/AKT pathway which can activate Cdk6 activity through upstream signaling events implicating Cdk6 in cellular responses to growth signals.
