Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:> 2 µM,
Range:2 - 200 µM,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.
In the aspartate assay protocol, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 μM).
This product is manufactured by BioVision, an Abcam company and was previously called K552 Aspartate Colorimetric/Fluorometric Assay Kit. K552-100 is the same size as the 100 test size of ab102512.
L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys).
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aspartate also known as aspartic acid is a non-essential amino acid with a molecular mass of 133.10 g/mol. It is an important component in proteins and is found widely expressed in various tissues throughout the body. Aspartate participates in several metabolic processes acting as a neurotransmitter in the central nervous system and playing roles in enzyme function as a medium for nitrogen transport and as a substrate in the urea cycle.
Biological function summary
Members of the broader aspartate family are important in several biochemical areas. Aspartic acid works as a precursor for the synthesis of other amino acids and nucleotides. It also forms part of aspartate transaminase an enzyme that contributes to the transfer of amino groups. In cellular metabolism aspartate integrates into the synthesis of purines and pyrimidines contributing to nucleotide formation which is critical for DNA and RNA production.
Pathways
The role of aspartate extends to significant metabolic routes. It participates in the citric acid cycle also known as the Krebs cycle where it can be converted into oxaloacetate. It also maps to the urea cycle where it acts to eliminate excess nitrogen from organisms. Through these pathways aspartate interacts with proteins such as fumarase and argininosuccinate synthase highlighting its connectivity to fundamental metabolism.
Abnormalities in aspartate levels can associate with specific medical conditions. For instance elevated aspartate transaminase may indicate liver disease as damaged liver cells release plentiful enzymes into the bloodstream. Furthermore aspartate dysregulation is linked to disorders like hyperammonemia where the protein's failure to connect to glutamate and urea cycle proteins such as ornithine transcarbamylase results in excess ammonia levels in the blood.
