Answer:No. It contains human thyroglobulin residues 2659–2768 with an N-terminal GST tag.
Answer:It is expressed in a wheat-germ system, with a predicted fusion-protein mass of about 37.84 kDa.
Answer:SDS-PAGE, ELISA, and western blotting; the supplied amount is 10 µg.
This recombinant human thyroglobulin fragment provides a defined antigen preparation for laboratory protein and antibody studies. The construct contains residues 2659–2768 of human thyroglobulin with an N-terminal GST tag and is expressed in a wheat-germ system. The predicted molecular weight of the fusion product is approximately 37.84 kDa. It is suited to assays such as ELISA, western blotting, and SDS-PAGE, including evaluation of antibodies that recognize epitopes within the included thyroglobulin region. The protein is a fragment, not native full-length thyroglobulin, and is not reported as biologically active. This package contains 10 µg. The GST fusion and limited sequence coverage should be considered when interpreting antibody binding or designing antigen controls.
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