Abcam, ab136947-1 x 96Tests, SMN ELISA kit

Name: SMN ELISA kit
Brand: Abcam
SKU: ab136947-1 x 96Tests

SMN ELISA kit is a sandwich ELISA used to quantify Survival Motor Neuron protein with a sensitivity of 50 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - wide measuring range: 50 - 3200 pg/mL

Catalog Number: ab136947-1 x 96Tests

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Category

Sandwich ELISA Kits

Pack Size1 x 96 Tests

Manufacturer's Pagewww.abcam.com/en-us/products/elisa-kits/smn-elisa-kit-ab136947

Description

Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts, Cell Lysate,
Reacts with:Mouse, Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 50 pg/mL,
Range:50 - 3200 pg/mL,
Assay Platform:Microplate

Product details:
SMN ELISA kit ab136947 is a sandwich ELISA to measure SMN in tissue Extracts, cell Lysate with a sensitivity of 50 pg/mL.
How the assay works:
An anti-Human SMN antibody is precoated onto 96-well plates, standards or test samples are added to the wells and incubated at room temperature. The wells are washed and a polyclonal detector antibody specific to SMN is added, followed by incubation at room temperature. After further washing, a horseradish peroxidase (HRP) conjugated anti-species antibody is added to each well and incubated at room temperature. After incubation the excess reagents are washed away. TMB substrate is added to each well and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 450 nm. The intensity of the yellow coloration is directly proportional to the amount of SMN captured in the plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
SMN ELISA kit ab136947 protocol summary
Add standard or sample to each well used. Incubate at room temperature.
2. Wash and add prepared detection antibody to each well. Incubate at room temperature.
3. Wash and add prepared antibody HRP conjugate. Incubate at room temperature.
4. Add TMB Substrate to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.
Get results in 90 minutes with our SimpleStep ELISA
& reg;
Human SMN/Gemin 1 ELISA Kit
ab315779
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMN also known as Survival of Motor Neuron protein or Gemin 1 is an important protein involved in the assembly of small nuclear ribonucleoproteins (snRNPs). It has a molecular mass of approximately 38 kDa. SMN proteins are expressed in almost all tissues with highest levels in spinal cord and brain tissues. They play a mechanical role in Gemin complex assisting RNA processing tasks which are essential in RNA metabolism alongside other Gemin proteins.
Biological function summary
The SMN protein operates within the SMN-Gemin complex a multi-protein assembly important for snRNP biogenesis and pre-mRNA splicing. This complex coordinates the maturation and transport of snRNPs facilitating the processing of precursor mRNAs in the nucleus. SMN and associated Gemin proteins mediate the assembly of Sm proteins onto snRNA ensuring accurate splicing mechanisms necessary for gene expression.
Pathways
The SMN protein is notably involved in the chaperone-assisted assembly pathway related to snRNP biogenesis. It plays a central role in the spliceosome assembly process working closely with Sm proteins and other RNA-binding proteins. Importantly SMN interacts with the ribonucleoprotein complex influencing mRNA splicing which integrates into the broader cell signaling pathways such as the cholinergic agonist pathways and RNA splicing pathways that regulate neuromuscular communication.
The deficiency or malfunction of SMN protein directly relates to Spinal Muscular Atrophy (SMA) an autosomal recessive disorder. SMA is characterized by motor neuron degeneration which can be attributed to insufficient SMN protein levels leading to compromised snRNP assembly and splicing. The SMN1 gene deletion or mutation reduces the available SMN protein linking this defect with the severity of SMA. Studies suggest that altering the expression levels of the related SMN2 gene can potentially modulate disease outcomes further emphasizing the connection between SMN protein and SMA pathogenesis.

FAQs

Question: Which samples are supported by the SMN ELISA kit?

Answer:It is designed to quantify SMN protein in human and mouse cell lysates or tissue extracts.

Question: What is the sensitivity and measuring range?

Answer:The listed sensitivity is 50 pg/mL, with a quantitative range of 50–3200 pg/mL.

Question: Does the assay measure SMN1 gene copy number?

Answer:No. It measures SMN protein concentration by sandwich ELISA and does not provide genetic copy-number or mutation analysis.

Application Note

The Abcam SMN sandwich ELISA kit measures survival motor neuron (SMN) protein in human and mouse cell lysates or tissue extracts. The 96-test colorimetric microplate format uses immobilized capture antibody, a detection antibody, enzyme-linked signal development and a 450 nm absorbance measurement to quantify SMN relative to a standard curve. The published assay measuring range is 50–3200 pg/mL, with a sensitivity of 50 pg/mL. Prepare lysates and tissue extracts using the assay-compatible extraction procedure, then dilute samples as needed to bring the expected SMN concentration within the working range. Include standards, blanks and appropriate biological controls on each plate. Wash thoroughly between incubations to control background, and evaluate sample dilution parallelism or recovery when testing new sample matrices. The assay measures protein abundance; it does not establish SMN gene copy number, mutation status or a clinical diagnosis. The kit is for research use and requires the current protocol for exact reagent preparation and incubation steps.

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