Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cell Lysate,
Assay type:Quantitative,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Cholesterol/Cholesteryl Ester Assay Kit ab102515 uses a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method.
This assay kit uses an alternative method compared to our most popular Cholesterol/ Cholesteryl Ester Assay Kit
ab65359
(which relies on a cholesterol oxidase based method).
Cholesterol assay principle
In this cholesterol assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm.
The assay can detect free or total cholesterol depending upon whether cholesterol esterase is used to hydrolyze cholesterol esters present.
Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters).
Cholesteryl assay protocol summary
- add samples and standard to wells
- add reaction mix and incubate for 30-min at 37°C
- analyse with plate reader
Other Notes
This product was previously called K623 Biovision Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II. Biovision was acquired by Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cholesterol/cholesteryl ester (CE) plays a mechanical role in the body by transporting lipids. Known also as 'cholesteryl esters' these molecules consist of cholesterol bound to long-chain fatty acids. CE exhibits a molecular mass that varies depending on the fatty acid chain length. These molecules are typically found within plasma and intracellular lipid droplets in tissues such as the liver and intestines. They mediate cholesterol transport and storage maintaining cellular lipid homeostasis.
Biological function summary
Cholesteryl esters facilitate cholesterol influx and efflux by serving as precursor molecules for various hormones and vitamin D. They do not exist in isolation but rather as part of larger lipid complexes including chylomicrons very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL). These lipid complexes play an essential role in delivering cholesterol to peripheral tissues and returning excess to the liver for excretion or reprocessing.
Pathways
Cholesteryl esters are involved in lipid metabolism processes such as the reverse cholesterol transport and the esterification of cholesterol through the action of lecithin-cholesterol acyltransferase (LCAT). This esterification process converts free cholesterol into cholesteryl esters facilitating their transport within lipoproteins. High-density lipoprotein (HDL) serves as a pathway partner circulating cholesteryl esters back to the liver where they undergo hydrolysis and re-utilization.
Cholesteryl esters are significant in the development of atherosclerosis and coronary artery disease. Dysregulation of CE metabolism can lead to the accumulation of lipid-rich plaques in blood vessel walls catalyzed by proteins like apolipoproteins such as ApoB-100 and ApoE. Excessive LDL and CE deposition in arterial walls are associated with plaque formation culminating in cardiovascular diseases. Understanding the role of these compounds and their associated pathways is important for developing treatments against lipid disorders.
