Description
Key facts
Detection method:Luminescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Sandwich (qualitative),
Sensitivity:< 10 Cells/well,
Assay time:2h,
Assay Platform:Microplate
Product details:
ab126572 involves
incorporation of BrdU into cells
cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture BrdU is added to wells of the microtiter plate. BrdU will be incorporated into the DNA of dividing cells. To enable
antibody binding to the incorporated BrdU cells
must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixing Solution.
Detector anti-BrdU monoclonal antibody
is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU. Unbound antibody is washed away and horseradish peroxidase-conjugated goat anti-mouse antibody is added, which binds to the Detector Antibody.
The horseradish peroxidase catalyzes the oxidation of diacylhydrazides. The reaction product, in its excited state, decays yielding light in the process. This reaction has a large dynamic range spanning many logs of relative luminescence. The intensity of the luminescence is proportional to the amount of incorporated BrdU in the cells. The reaction is quantified using a luminometer and is plotted as relative light units per second (RLU/s).
The resultant assay is sensitive, rapid, easy to perform and applicable to high sample throughput
. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
