Description
Key facts
Detection method:Fluorescent,
Assay Platform:Flow cytometer,
Sample types:Suspension cells, Adherent cells,
Assay type:Sandwich,
Reacts with:Human
Product details:
Hypoxia and the cellular response to hypoxic environment are central topics in studies of metabolism, cancer progression and development and stem cells. A key player is the transcription factor HIF1 alpha (hypoxia inducible factor 1 alpha) which is stabilized at the protein level in response to decreased oxygen tension. HIF1 alpha then promotes transcription of a number of factors that alters cellular physiology. This Hypoxic Response Human Flow Cytometry Kit provides duplexed measurements of the transcription factor HIF1 alpha and the HIF1 alpha responsive protein BNIP3.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIF1a also known as Hypoxia-Inducible Factor 1-alpha is a transcription factor with a mass of approximately 92 kDa. It functions mainly in response to low oxygen levels (hypoxia) within cells. HIF1a exists in the cytoplasm under normal oxygen conditions and translocates to the nucleus during hypoxia. It is expressed in various tissues and cells particularly those located in areas with fluctuating oxygen levels such as skeletal muscle and heart tissues. The protein also shows up in less oxygenated environments like tumors due to its role in adapting to and surviving low oxygen conditions.
Biological function summary
HIF1a plays a major role in oxygen homeostasis by regulating the expression of genes involved in important processes such as angiogenesis metabolism and cell survival. HIF1a forms a heterodimeric complex with HIF1b enabling DNA binding to hypoxia response elements of target genes. Additionally BNIP3 an apoptosis-inducing protein regulated by HIF1a under hypoxic conditions promotes cell death and autophagy. The interplay between HIF1a and BNIP3 contributes to maintaining cellular energy balance and responding to stress.
Pathways
HIF1a is an important player in the hypoxia response pathway which involves numerous transcriptional targets that control oxygen-sensitive processes. It tightly associates with the VEGF (vascular endothelial growth factor) pathway to promote angiogenesis. HIF1a also links with other proteins like PHDs (prolyl hydroxylases) which play a part in the oxygen-sensing mechanism by regulating the degradation of HIF1a under normoxic conditions. Through these pathways HIF1a ensures cells adapt to and survive fluctuations in oxygen availability.
HIF1a has significant roles in cancer and cardiovascular diseases due to its involvement in promoting cell survival angiogenesis and metabolic reprogramming. Elevated levels of HIF1a are commonly found in many tumors where it drives tumor progression through enhanced glucose metabolism and blood vessel formation. In cardiac ischemia HIF1a activation aids tissue adaptation to oxygen deprivation and may protect against heart failure. Its role in both cancer and cardiovascular disease often links with BNIP3 where it contributes to disease pathology by modulating programmed cell death and adaptation mechanisms.
