Description
Key facts
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay Platform:Flow cytometer, Fluorescence microscope
Product details:
Cellular Superoxide Detection Assay Kit (ab139477) is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with standard orange (e.g. 550/620 nm) or red (e.g. 650/670 nm) fluorescent cubes, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser.
The Cellular Superoxide Detection Assay kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension).
**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
