Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Cell culture extracts, Suspension cells, Tissue Extracts, Adherent cells,
Assay type:Quantitative,
Assay Platform:Microplate reader
Product details:
Abcam's 2-Phosphoglycerate Assay kit (ab174097) is a sensitive, fast and easy-to-use kit. In this assay, 2PG(2-phosphoglycerate) is converted by Enzyme Mix to PEP (phosphoenolpyruvate), which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
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This product is manufactured by BioVision, an Abcam company and was previously called K778 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit. K778-100 is the same size as the 100 test size of ab174097.
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2PG is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
2-Phosphoglycerate (2-PG) also known as phosphoglycerate is an intermediate metabolite in the glycolytic pathway. It results from the conversion of 3-phosphoglycerate to 2-phosphoglycerate by the enzyme phosphoglycerate mutase. It then converts into phosphoenolpyruvate (PEP) under the action of enolase an enzyme important for this transformation. 2-Phosphoglycerate is small in mass and expressively found in cellular cytoplasm where glycolysis takes place.
Biological function summary
2-phosphoglycerate plays a role in energy metabolism as an integral part of the glycolytic pathway. It exists within the cellular environment facilitating cellular respiration by progressing the glycolytic pathway towards the generation of pyruvate. It does not form part of a large protein complex functioning independently to ensure progression through glycolysis.
Pathways
2-phosphoglycerate is mainly involved in the glycolysis pathway that helps in the breakdown of glucose to pyruvate for energy production. The transformation from 2-PG to PEP via enolase signifies its role in energy yield within cells. It is closely associated with proteins like enolase and phosphoglycerate mutase which directly interact with it in the metabolic pathway sequence.
Disturbances in the equilibrium of 2-phosphoglycerate levels can contribute to metabolic disorders such as glycolytic enzyme deficiencies. While rare these enzyme deficiencies can impact energy metabolism resulting in conditions like hemolytic anemia. Alterations in the glycolytic pathway involving this metabolite could affect the function of enzymes like enolase which may have further consequences in energy-related disorders.
