Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (qualitative),
Assay Platform:Microplate
Product details:
Abcam's anti-Chikungunya Virus IgM Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Chikungunya Virus in Human serum and plasma.
A 96-well plate has been precoated with anti-Human antibodies to bind to corresponding antibodies of the sample. Controls or test samples are added to the wells and incubated. Following washing, the Chikungunya Virus antigen is added to the wells and incubated. The wells are then washed as before and the biotinylated Chikungunya Virus antibodies are added to each well followed by incubation. After a last wash step, the streptavidin peroxidase (SP) conjugate which binds to the biotinylated Chikungunya Virus-specific antibodies is added to the wells. TMB is then catalyzed by the SP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chikungunya Virus IgM sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
