Description
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its binding ability in a functional ELISA. Immobilized Human GH, Tag Free at 2μg/mL (100 μL/well) can bind Human GHR, His Tag (ab180053) with a linear range of 1-8 ng/mL (QC tested).,
Accession:P10912,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. To avoid surface adsorption loss and inactivation the reconstituted protein must not be aliquoted to less than 10 µg per vial.,
Storage buffer:pH: 7.4Constituents: PBS, 10% Trehalose
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Growth Hormone Receptor (GHR) also known as somatotropin receptor is a vital protein that mediates the effects of growth hormone (GH). GHR is a transmembrane receptor with a mass of approximately 70 kDa and is expressed in many tissues including liver muscle and adipose tissue. It plays an important role in growth regulation by binding to growth hormone which triggers signal transduction pathways involved in cellular proliferation and growth.
Biological function summary
The growth hormone receptor facilitates the hormone's ability to promote growth and cell differentiation. The receptor when activated stimulates the JAK2/STAT5 signaling pathway a part of a larger GHR complex. This complex leads to gene transcription that stimulates growth and metabolism. The activation of GHR has significant effects on the cellular functions related to protein synthesis lipid metabolism and glucose metabolism.
Pathways
The growth hormone receptor is an important component of the JAK-STAT signaling pathway and the mTOR signaling pathway. In these pathways GHR interacts with other proteins such as Janus kinase 2 (JAK2) and signal transducer and activator of transcription 5 (STAT5). These interactions play a critical role in translating the binding of growth hormone into cellular responses that lead to growth and metabolic regulation.
Disruptions or mutations in the growth hormone receptor can lead to conditions such as Laron syndrome and growth hormone insensitivity. These conditions often display symptoms of dwarfism and metabolic aberrations due to impaired signaling. GHR malfunction relates closely to growth hormone (GH) and anti-growth hormone treatments like somatropin used for managing growth deficiencies. The connection of GHR with related pathways and proteins like the IGF-1 (Insulin-like Growth Factor 1) underlines its role in metabolic diseases.
