Description
Key facts
Applications:IP, PurificationSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.
Product details:
Contents:
Supplied as 50% slurry in 20% Ethanol.
Features:
High binding capacity: Binding of IgG >20 mg human or rabbit IgG/mL Protein G Sepharose
Minimal Leaching of the Ligand
Flow Rate Tested*: 2.07 mL/min.
*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Usage: Reusable for up to 10 times without significant loss of binding capacity.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x
when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Sepharose is a registered trademark of GE Healthcare
This product is manufactured by BioVision, an Abcam company and was previously called 6511 Protein G Sepharose. 6511-100 is the same size as the 100 ml size of ab193259.
Protein G Sepharose
beads are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose
beads. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G Sepharose
is >20 mg of human or rabbit IgG per mL of wet beads. Protein G Sepharose
beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate long-term storage conditions-+4°C
