Description
Key facts
Applications:PurificationSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:Constituents: 80% PBS, 20% Ethanol
Product details:
Contents:
Supplied as a 50 % slurry in 20 % Ethanol / PBS; > 3 mg (10 μmol) glutathione per mL Sepharose
beads.
Features:
The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the overexpression of foreign genes in
E. Coli
and other expression systems. The GST-tagged protein can be easily purified from cell lysate or media by affinity chromatography using glutathione Sepharose
beads. Abcam's Glutathione Sepharose
is designed for the purification of GST fusion proteins and other glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x
when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of GST-fusion proteins or other glutathione-binding proteins.
Sepharose is a registered trademark of GE Healthcare
This product is manufactured by BioVision, an Abcam company and was previously called 6555 Glutathione Sepharose. 6555-10 is the same size as the 10 ml size of ab193267.
Glutathione Sepharose
is prepared by covalently coupling glutathione to epoxy-activated 4% cross-linked Sepharose
beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity, which could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate long-term storage conditions-+4°C
