Abcam, ab201117-1Kit, Protease Inhibitor Cocktail III

Product details:
Abcam’s protease inhibitor cocktails inhibit a variety of endogenous proteases to protect your protein samples from degradation.
Ideal for bacterial cell extracts using metal chelation chromatography.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases, aspartic proteases
Inhibitors included - AEBSF hydrochloride, bestatin, E-64, EDTA disodium salt, pepstatin A
Supplied as 1 vial of lyophilised cocktail and 1 vial of DMSO (1 ml)
Preparation:
Reconstitute with 1 ml DMSO and 4 ml DI water to obtain a 5 ml solution
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Individual components and concentrations:
AEBSF hydrochloride:
serine protease inhibitor (20 mM)
Bestatin:
aminopeptidase B and leucine aminopeptidase inhibitor (1.7 mM)
cysteine protease inhibitor (0.2 mM)
EDTA disodium salt:
metalloprotease inhibitor (85 mM)
Pepstatin A:
aspartic protease inhibitor (2 mM)

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate long-term storage conditions--20°C, Storage information--20°C