Description
Key facts
Detection method:Fluorescent,
Sample types:Purified protein, Plasma,
Assay type:Enzyme activity,
Sensitivity:< 1 ng,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Factor Xa Activity Assay Kit (Fluorometric) (ab204711) utilizes the ability of Factor Xa to cleave a synthetic substrate thereby releasing a fluorophore, AMC, which can be quantified by fluorescence readers. This assay kit is simple, rapid and can detect Factor Xa activity as low as 1 ng.
Factor Xa activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader for 30-60 min every 2-3 min
For plasma samples, citrate plasma or heparin plasma is recommended as EDTA may interfere with the enzymatic assay.
This product is manufactured by BioVision, an Abcam company and was previously called K361 Factor Xa Activity Fluorometric Assay Kit. K361-100 is the same size as the 100 test size of ab204711.
Factor Xa (FXa) is the activated form of the coagulation factor X (Stuart-Power factor, thrombokinase, prothrombinase, thromboplastin, E.C.3.4.21.6). Factor X, a serine endopeptidase plays an important role at several stages of the coagulation pathway. It acts by converting prothrombin into active thrombin by complexing with activated co-factor V in the prothrombinase complex. Unfractionated heparin and various low molecular weight heparins bind to plasma cofactor antithrombin to inactivate several coagulation factors including factor Xa.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor Xa also known as activated factor X is an important serine protease in the coagulation cascade. With a molecular mass around 37 kDa this enzyme is an important component in blood coagulation. Factor Xa derives from the hepatic synthesis of factor X requiring vitamin K for its gamma-carboxylation and it is found circulating within the blood plasma. The conversion of factor X into factor Xa occurs when exposed to either extrinsic or intrinsic coagulation pathways.
Biological function summary
Activated factor X plays a pivotal role in converting prothrombin into thrombin facilitating the cross-linking of fibrin which stabilizes the forming clot. Factor Xa while acting as a monomer often associates with factor Va to form prothrombinase complex enhancing its efficiency. The prothrombinase complex accelerates thrombin generation exponentially which is an essential step in hemostasis.
Pathways
The blood coagulation cascade involves factor Xa prominently within the intrinsic and extrinsic pathways. Factor Xa lies downstream in these pathways and links them to the common pathway. It directly interacts with thrombin and factor Va forming an integral part of both pathways in the conversion of prothrombin to thrombin. These steps are important in maintaining hemostasis and enabling proper clot formation during vascular injury.
Factor Xa is highly relevant in coagulopathies such as deep vein thrombosis (DVT) and pulmonary embolism (PE). Aberrant activity of this factor contributes to excessive clot formation leading to thrombotic disorders. Additionally factor Xa is a target for anticoagulant therapies such as rivaroxaban and apixaban which inhibit its activity to prevent thrombotic events. Such inhibitors affect conditions related to factor Xa impacting the risk and management of cardiovascular diseases.
