Description
Key facts
Detection method:Fluorescent,
Sample types:Urine, Plasma, Tissue Extracts, Cell Lysate,
Assay type:Quantitative,
Sensitivity:= 10 nM,
Assay time:30m,
Assay Platform:Microplate reader
Product details:
How the assay works
The GSH/GSSG assay protocol uses a proprietary non-fluorescent water-soluble dye that becomes strongly fluorescent upon reacting with GSH. With a one-step fluorimetric method, the kit can detect as little as 1 picomole of GSH or GSSG in a 100 µL assay volume.
The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm.
GSH/GSSG assay protocol summary:
- add samples (deproteinized) and standards to wells
- for GSH assay add Thiol Green in assay buffer, or for total glutathione (GSH + GSSG) assay also add GSSG probe
- incubate for 10 - 60 min at room temp
- analyze with microplate reader
GSSG levels can be calculated by subtracting GSH from total glutathione levels.
NOTE: For measuring GSH Standard only, there is enough reagent provided to perform 200 tests.
How other researchers are using
GSH/GSSG Ratio Detection Assay Kit II (Fluorometric - Green) has been used in a variety of sample type including:
- Human umbilical cord mesenchymal stem cells (hUCMSCs) 1
- Mouse kidney tissues 2
- Rat Brain homogenates 3
References:
1 - Hu B et al. 2023; 2 - Gong Q et al. 2023; 3 - Fuchs M et al. 2023.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
