Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts, Cell culture supernatant,
Reacts with:Mouse, Rat, Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5 pg/mL,
Range:78.13 - 5000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)
Product details:
AKT2 ELISA Kit (ab208986) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AKT2 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate AKT2 with 5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases. These kinases regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Specifically, AKT2 seems to be the principal isoform responsible for the regulation of glucose uptake. The AKT kinases mediate these processes through serine and/or threonine phosphorylation of a range of downstream substrates.
Human and rat AKT2 exhibit 98.1% and 98.5% amino acid identity to mouse AKT2, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The AKT2 protein also known as protein kinase B beta is a serine/threonine kinase with a mass of approximately 56 kDa. It plays a critical mechanical role in cell survival and cell growth. AKT2 is an isoform of the AKT family and shares structural similarity with other AKT proteins. It is widely expressed in various tissues with high levels in insulin-responsive tissues such as skeletal muscle adipose tissue and liver. The AKT2 protein is an essential component in transmitting cellular signals related to growth and metabolism.
Biological function summary
The protein engages in controlling glucose transport and metabolism. As part of its biological functions AKT2 becomes phosphorylated and activated in response to insulin signaling and does not work as a part of a larger complex but interacts with other proteins. It plays a significant role in maintaining normal insulin sensitivity in tissues by facilitating the uptake and storage of glucose. Additionally the protein influences cell cycle progression and can affect apoptotic responses contributing to processes like protein synthesis and cell proliferation.
Pathways
AKT2 involves signaling mechanisms that include the PI3K/AKT/mTOR pathway and the insulin signaling pathway. These pathways are deeply connected to cellular growth and metabolic regulation. AKT2 works closely with proteins such as PI3K which phosphorylates phosphoinositides and mTOR that control protein synthesis by activating downstream targets. These interactions highlight the integration of AKT2 in nutrient-sensing pathways and how it aids the regulation of energy status in response to external stimuli.
AKT2 is notably linked to conditions such as type 2 diabetes and cancer. These diseases often involve dysregulated signaling pathways where AKT2 plays a significant role. In type 2 diabetes AKT2's link to insulin signaling becomes critical and its aberrant activity can lead to insulin resistance. In cancer the AKT2 protein interacts with PTEN a tumor suppressor that negatively regulates the PI3K/AKT pathway often leading to unregulated cell growth when mutated or inhibited. The understanding of AKT2's involvement in these diseases assists in developing potential therapeutic strategies targeting its dysregulation.
