Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.1 pg/mL,
Range:4.69 - 300 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
HIV1 p24 ELISA Kit ab218268 is a rapid single-wash 90-min ELISA kit to measure HIV1 p24 in cell culture extracts, tissue extracts, serum and plasma. This SimpleStep ELISA® sensitivity is 1.1 pg/mL.
How the assay works
HIV1 p24 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our HIV1 p24 SimpleStep ELISA® can be used to rapidly and sensitively determine the titre of HIV1 particles in range of sample types.
HIV1 p24 ELISA Kit ab218268 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using HIV1 p24 ELISA Kit ab218268:
HIV1 p24 ELISA Kit ab218268 has been used in publications to study HIV-1 infection and therapeutic approaches to AIDS
(1)(2)
References:
(1)Kai Deng et al. 2021 PMID: 33835029,
Vir B Singh et al. 2023 PMID: 37663574
Related and recommended products
HIV1 p24 ELISA Kit ab218268 is commonly used to study lentivirus vectors based on HIV-1.
Other products for analyzing lentiviruses include:
- Mounting Medium with DAPI - Aqueos, Fluoroshield
ab104139
- Goat Anti-Mouse IgG H&L (HRP)
ab205719
HIV1 p24 (capsid) protein is essential for HIV-1 viral replication and for the HIV-1 infection of non-dividing cells. HIV1 p24 proteins form viral capsid that encapsulates the genomic HIV1 RNA. HIV1 p24 concentration in host plasma is commonly used as indicator of viral load. Upon the viral infection, the development of anti-HIV1 p24 host humoral responses leads to immune complex formation and reduction of the free HIV1 p24 concentration in circulation.
Lentivirus vectors based on HIV-1 have gained popularity as a tool for cell and gene therapy, owing to their ability to carry and integrate a high volume of transgenes in a range of dividing and non-dividing cell types. Pseudo-lentiviral particles are typically produced from 293T cells and harvested from the medium 48-78hrs post-transfection. To ensure that the pseudoviral medium is viable, and to control the number of copies of integrated viral constructs per target cell, the viral titre needs to be determined before transduction experiments. Our HIV1 p24 SimpleStep ELISA
can be used to rapidly and sensitively determine the titre of HIV1 particles in range of sample types.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p24 protein also known as the HIV-1 capsid protein or CA is an important component of the Human Immunodeficiency Virus Type 1 (HIV-1). It has a molecular weight of approximately 24 kDa. This protein is expressed in the viral core and plays a critical role in the virus's structural integrity. The p24 protein helps form the conical core that encases the viral RNA genome. Antibody-based detection of this protein is a common approach to study HIV often utilizing tools such as the p24 ELISA kit and p24 assay which are pivotal in early HIV detection and research.
Biological function summary
The p24 protein is essential in the assembly and maturation of the HIV-1 virion. It forms part of the Gag polyprotein precursor which undergoes proteolytic cleavage to create the mature viral proteins necessary for the formation of infectious particles. The Gag polyprotein assembles into the immature virion and later cleaves into functional units including the matrix capsid and nucleocapsid proteins. This process ensures the viral core is capable of delivering the genetic material to the host cell during infection. Such assembly is important for maintaining the infective efficiency of the HIV-1 virus.
Pathways
P24 plays an integral role in the lifecycle of HIV-1 especially in the virus replication and assembly pathways. It interacts with host cellular machinery to facilitate the viral assembly and is involved in the highly coordinated regulation of the viral life cycle. This protein shares pathway connections with other viral proteins such as p55 Gag and matrix protein p17 both of which also derive from the Gag polyprotein. These interactions underline the importance of p24 in the broader context of HIV-1's ability to propagate within host cells.
The p24 protein is directly linked to the pathogenesis of Acquired Immunodeficiency Syndrome (AIDS). Understanding and detecting the presence of p24 are vital for diagnosing and monitoring the progression of HIV-1 infection. Additionally it holds a significant relationship with immune system disorders due to the virus's effect on immune function. p24 detection is often a component in HIV ELISA and HIV p24 ELISA tests which measure the presence of the virus in the bloodstream. The protein also connects to the envelope glycoprotein gp120 which is involved in initial host cell binding and viral entry furthering its pivotal role in the progression of HIV-related diseases.
