Description
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 30.34 pg/mL,
Range:62.5 - 4000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Apo E ELISA Kit ab233623 is a rapid single-wash 90-min ELISA kit to measure Human Apo E in milk, serum and plasma. This SimpleStep ELISA® sensitivity is 30.34 pg/mL.
How the assay works
Human Apo E SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Apo E protein in serum, plasma, and milk samples only.
Human Apo E ELISA Kit ab233623 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Apo E ELISA Kit ab233623
Human Apo E ELISA Kit ab233623 has been used to develop screening tools for Alzheimer's Disease
, to study LDL interactions after bariatric surgery
, and to evaluate the role of ApoE in rheumatoid arthritis
References:
Seungjoo Haam et al. 2023, PMID: 38071202,
Olga Gursky et al. 2023, PMID: 37777014,
Anna M Blom et al. 2020, PMID: 32253242
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Apo E Antibody Pair - BSA and Azide free
ab244096
- Anti-Apolipoprotein E antibody [EPR19863-372] - BSA and Azide free (Capture)
ab244739
- Anti-Apolipoprotein E antibody [EPR19863-383] - BSA and Azide free (Detector)
ab245008
Related and recommended products
Human Apo E ELISA Kit ab233623 is commonly used to study lipid metabolism and neurodegenerative diseases.
Other kits to measure lipid metabolism and Alzheimer's Disease include:
- Human Apolipoprotein AI ELISA kit
ab189576
- Human Apolipoprotein B ELISA kit
ab190806
- Human Clusterin ELISA kit
ab174447
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.
Biological function summary
ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.
Pathways
In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.
In Alzheimer’s disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.
