Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay Platform:Microplate reader
Product details:
Cell Migration/Chemotaxis Assay Kit (96-well, 8 μm) (ab235673) measures cell migration in response to stimuli in adherent and suspension cells. It allows you to screen, study, or characterize compounds that influence chemotaxis/cell migration. It utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.
8 μm semi-permeable membrane maybe too large to use with tiny cell types, such as cell types with diameter less than 8 μm.
Cell migration / chemotaxis assay protocol summary
- Grow cells.
- Add cell culture media containing desired chemoattractant to the bottom part of the Boyden chamber (bottom chamber).
- Add cell culture media containing positive control Migration Inducer to control wells in the bottom chamber.
- Add cells to the top part of the Boyden chamber (top chamber) with any desired treatment.
- Incubate at 37°C in cell culture incubator for 2-48 h.
- Cells will migrate through the polycarbonate membrane from the top chamber to the bottom chamber.
- Remove the top chamber and remove media and remaining cells.
- Centrifuge the bottom chamber and wash cells with Wash Buffer, repeat.
- Add Cell Dye / Cell Disassociation Solution to bottom chamber and incubate for 60-min in cell culture incubator.
- Analyze with fluorometric (Ex/Em 530/590 nm) plate reader.
- Prepare a separate standard curve with known numbers of cells in a 96-well plate with Cell Dye, incubate for 1-hr, and analyze with fluorometric (Ex/Em 530/590 nm) plate reader to allow quantitation.
Other notes
This product was previously called K906 Biovision EZCell™ Cell Migration/Chemotaxis Assay Kit (96-well, 8 μm). Biovision was acquired by Abcam in 2021.
Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical and mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
