Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Urine, Plasma, Serum, Cell Lysate
Product details:
The Homocitrulline/Citrulline Assay Kit (ab242292) provides a convenient colorimetric method for the detection of total homocitrulline/citrulline from cells, tissue, plasma, serum, or urine samples.
The content of homocitrulline and citrulline in the unknown samples is determined by comparison with a predetermined standard curve. The provided reagents are sufficient for the evaluation of 100 assays including standards and unknown samples.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Citrulline also known as L-citrulline engages in important roles within the nitrogen balance of the body. It is a non-essential amino acid with a molecular weight of about 175.19 g/mol. Citrulline is not incorporated into proteins but is primarily found in liver and intestinal cells. It serves as an intermediate in the urea cycle an important metabolic pathway related to the disposal of excess nitrogen from the body.
Biological function summary
Citrulline plays a significant part in the body's nitrogen cycle and enhances the production of nitric oxide a vital signaling molecule. Citrulline participates in converting ammonia a byproduct from amino acid breakdown into urea in the liver which the body can then excrete. It does not belong to a complex but acts as a precursor to the amino acid arginine enhancing its levels and promoting vasodilation. Citrulline effects also extend to influencing muscle metabolism and cellular energy production.
Pathways
Citrulline participates in the urea cycle and nitric oxide synthesis pathway. It provides a link between ammonia detoxification and the synthesis of arginine. This link enhances nitric oxide availability which is essential for regulating vascular homeostasis and blood pressure. Additionally citrulline relates to the arginine biosynthesis pathway where it aids the conversion to arginine mediated by enzymes such as argininosuccinate synthetase and argininosuccinate lyase. These interactions with enzymes highlight its integration into metabolic pathways central to the organism's homeostasis.
Citrulline shows involvement in cardiovascular diseases and urea cycle disorders. Citrulline has emerged as a therapeutic marker for cardiovascular diseases by enhancing nitric oxide production and improving endothelial function. Citrulline's role in urea cycle disorders links it to conditions where the body's ability to detoxify ammonia is compromised. Furthermore citrulline's relationship with arginine impacts both cardiac health and ammonia detoxification integrating it into therapeutic strategies targeting these conditions.
