Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon2 and 1 bp deletion in exon2 and 1 bp insertion in exon2.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-IFI16, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The IFI16 protein also known as Interferon γ Inducible Protein 16 is a DNA sensor belonging to the PYHIN family with a molecular mass of approximately 85 kDa. Widely expressed in the nucleus and cytoplasm its primary role involves detecting foreign DNA within cells. It possesses a pyrin domain two HIN-200 domains and also plays a role in regulating gene expression particularly in response to pathogen infection. This versatile protein can act in multiple cellular environments and its location within the cell can dictate its function.
Biological function summary
IFI16 protein participates in the innate immune response by recognizing viral and bacterial double-stranded DNA. It forms part of a signaling complex that activates pathogen recognition receptors and induces the production of type I interferons. IFI16 can interact with various DNA sensors and is known for its ability to bind DNA directly providing a defense mechanism against infection. The protein also plays a role in the regulation of transcription which contributes to cell growth and differentiation.
Pathways
IFI16 protein has significant roles in the cGAS-STING and BRCA1 pathways. It interacts with cGAS to regulate the production of cyclic GMP-AMP activating STING and promoting antiviral responses. Additionally IFI16 aligns with BRCA1 dysfunction impacting DNA repair processes. This interaction underlines its involvement in maintaining genomic integrity and modulating the immune responses along with cGAS STING and BRCA1 proteins which are critical in these pathways.
IFI16 protein plays a role in autoimmune diseases and various cancers. In Systemic Lupus Erythematosus (SLE) it can induce inflammation by recognizing self-DNA leading to chronic immune activation. In certain cancers its expression levels correlate with malignancy where it influences tumor progression and response to therapy. In these contexts IFI16 interacts closely with cGAS and BRCA1 proteins highlighting its relevance to disease mechanisms and offering potential therapeutic targets.
