Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon12 and 40 bp deletion in exon12 and 4 bp deletion in exon12.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-JAG2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Jagged 2 (JAG2) also known as JAGGED 2 is a membrane-bound ligand that weighs about 135 kDa. It interacts with the Notch family receptors to initiate signaling events that regulate cell fate decisions. JAG2 expresses in various tissues with high levels in neural hematopoietic and muscular systems. Its mechanical function involves binding to the Notch receptor on adjacent cells facilitating proteolytic cleavage and activation of the receptor's intracellular domain which then translocates to the nucleus to influence gene expression.
Biological function summary
The JAG2 protein plays a significant role in development and differentiation processes. It acts as a ligand in the Notch signaling pathway which includes other ligands like Jagged 1 (JAG1) and Delta-like proteins. In this complex JAG2 modulates neurogenesis myogenesis and angiogenesis. By affecting these processes JAG2 contributes to tissue homeostasis and the regulation of stem cells.
Pathways
The Notch signaling pathway involving JAG2 plays an important role in cell-cell communication. JAG2 activity in this pathway impact pathways like T-cell development and bone remodeling. It interacts with proteins like Notch1 and RBP-Jκ forming a pivotal part of the regulatory circuit that manages cell differentiation and proliferation.
Researchers link JAG2 to various conditions notably cancer and skeletal disorders. Dysregulation of JAG2 in the Notch pathway can contribute to oncogenesis with increased JAG2 expression observed in certain breast cancers. Additionally interactions between JAG2 and the Notch receptors influence bone diseases as alterations in signaling affect bone density and structure. Understanding the role of JAG2 and related proteins in these pathways allows potential therapeutic targeting in these conditions.
