Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon49.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-LAMA3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Laminin subunit alpha-3 also known by the gene name LAMA3 is a critical component of the extracellular matrix. The protein has a molecular weight of approximately 400 kDa. LAMA3 is primarily expressed in epithelial tissues contributing significantly to the structural integrity of basement membranes. The protein often partners with other laminin subunits to form different laminin isoforms which are essential for cellular adhesion and signaling.
Biological function summary
LAMA3 plays an important role in the formation of stable basement membranes and interacts with other proteins to maintain tissue architecture. As part of the laminin-332 complex alongside beta and gamma laminin chains LAMA3 ensures cell adhesion migration and differentiation processes. Its expression in tissues like skin and mucosal surfaces highlights its function in providing structural support and cellular microenvironment regulation.
Pathways
Laminin subunit alpha-3 is essential in pathways related to cell adhesion and migration such as integrin signaling and epithelial-mesenchymal transition. These pathways are pivotal during wound healing and tissue regeneration. LAMA3 associates with integrins particularly beta-1 and beta-4 integrins facilitating communication between cells and their extracellular environment. The interaction with integrins enables cells to respond to structural changes and external stimuli.
Mutations or alterations in LAMA3 can lead to conditions such as Epidermolysis Bullosa and Junctional Epidermolysis Bullosa where the integrity of the skin and epithelial tissues is compromised. Laminin subunit gamma-2 often interacts with LAMA3 and anomalies in this partnership contribute to these severe blistering disorders. Understanding the link between LAMA3 and these diseases helps inform the development of targeted therapies aimed at restoring normal cell-matrix interactions.
